TY - JOUR T1 - COPD-derived fibroblasts secrete higher levels of senescence-associated secretory phenotype proteins JF - Thorax JO - Thorax SP - 508 LP - 511 DO - 10.1136/thoraxjnl-2020-215114 VL - 76 IS - 5 AU - Roy R Woldhuis AU - Irene H Heijink AU - Maarten van den Berge AU - Wim Timens AU - Brian G G Oliver AU - Maaike de Vries AU - Corry-Anke Brandsma Y1 - 2021/05/01 UR - http://thorax.bmj.com/content/76/5/508.abstract N2 - COPD-derived fibroblasts have increased cellular senescence. Senescent cell accumulation can induce tissue dysfunction by their senescence-associated secretory phenotype (SASP). We aimed to determine the SASP of senescent fibroblasts and COPD-derived lung fibroblasts, including severe, early-onset (SEO)-COPD. SASP protein secretion was measured after paraquat-induced senescence in lung fibroblasts using Olink Proteomics and compared between (SEO-)COPD-derived and control-derived fibroblasts. We identified 124 SASP proteins of senescent lung fibroblasts, of which 42 were secreted at higher levels by COPD-derived fibroblasts and 35 by SEO-COPD-derived fibroblasts compared with controls. Interestingly, the (SEO-)COPD-associated SASP included proteins involved in chronic inflammation, which may contribute to (SEO-)COPD pathogenesis. ER -