RT Journal Article
SR Electronic
T1 Variation in oral microbiome is associated with future risk of lung cancer among never-smokers
JF Thorax
JO Thorax
FD BMJ Publishing Group Ltd and British Thoracic Society
SP 256
OP 263
DO 10.1136/thoraxjnl-2020-215542
VO 76
IS 3
A1 H Dean Hosgood
A1 Qiuyin Cai
A1 Xing Hua
A1 Jirong Long
A1 Jianxin Shi
A1 Yunhu Wan
A1 Yaohua Yang
A1 Christian Abnet
A1 Bryan A Bassig
A1 Wei Hu
A1 Bu-Tian Ji
A1 Madelyn Klugman
A1 Yongbing Xiang
A1 Yu-Tang Gao
A1 Jason YY Wong
A1 Wei Zheng
A1 Nathaniel Rothman
A1 Xiao-Ou Shu
A1 Qing Lan
YR 2021
UL http://thorax.bmj.com/content/76/3/256.abstract
AB Objective To prospectively investigate whether diversity in oral microbiota is associated with risk of lung cancer among never-smokers.Design and setting A nested case–control study within two prospective cohort studies, the Shanghai Women’s Health Study (n=74 941) and the Shanghai Men’s Health Study (n=61 480).Participants Lifetime never-smokers who had no cancer at baseline. Cases were subjects who were diagnosed with incident lung cancer (n=114) and were matched 1:1 with controls on sex, age (≤2 years), date (≤30 days) and time (morning/afternoon) of sample collection, antibiotic use during the week before sample collection (yes/no) and menopausal status (for women).Main outcomes and measures Metagenomic shotgun sequencing was used to measure the community structure and abundance of the oral microbiome in pre-diagnostic oral rinse samples of each case and control. Multivariable logistic regression models were used to estimate the association of lung cancer risk with alpha diversity metrics and relative abundance of taxa. The Microbiome Regression-Based Kernel Association Test (MiRKAT) evaluated the association between risk and the microbiome beta diversity.Results Subjects with lower microbiota alpha diversity had an increased risk of lung cancer compared with those with higher microbial alpha diversity (Shannon: ptrend=0.05; Simpson: ptrend=0.04; Observed Species: ptrend=0.64). No case–control differences were apparent for beta diversity (pMiRKAT=0.30). After accounting for multiple comparisons, a greater abundance of Spirochaetia (ORlow 1.00 (reference), ORmedium 0.61 (95% CI 0.32 to 1.18), ORhigh 0.42 (95% CI 0.21 to 0.85)) and Bacteroidetes (ORlow 1.00 (reference), ORmedium 0.66 (95% CI 0.35 to 1.25), ORhigh 0.31 (95% CI 0.15 to 0.64)) was associated with a decreased risk of lung cancer, while a greater abundance of the Bacilli class (ORlow 1.00 (reference), ORmedium 1.49 (95% CI 0.73 to 3.08), ORhigh 2.40 (95% CI 1.18 to 4.87)) and Lactobacillales order (ORlow 1.00 (reference), ORmedium 2.15 (95% CI 1.03 to 4.47), ORhigh 3.26 (95% CI 1.58 to 6.70)) was associated with an increased risk of lung cancer.Conclusions Our prospective study of never-smokers suggests that lower alpha diversity was associated with a greater risk of lung cancer and the abundance of certain specific taxa was associated with altered risk, providing further insight into the aetiology of lung cancer in the absence of active tobacco smoking.