PT - JOURNAL ARTICLE AU - Neda Farahi AU - Daniel Gillett AU - Mark Southwood AU - Doris Rassl AU - Nicola Tregay AU - Uta Hill AU - Stephen Denis Preston AU - Chrystalla Loutsios AU - Laurence Si Chong Lok AU - Sarah Heard AU - John Buscombe AU - Robert Campbell Rintoul AU - A Michael Peters AU - Charlotte Summers AU - Edwin R Chilvers TI - Lesson of the month: novel method to quantify neutrophil uptake in early lung cancer using SPECT-CT AID - 10.1136/thoraxjnl-2020-214642 DP - 2020 Sep 04 TA - Thorax PG - thoraxjnl-2020-214642 4099 - http://thorax.bmj.com/content/early/2020/09/03/thoraxjnl-2020-214642.short 4100 - http://thorax.bmj.com/content/early/2020/09/03/thoraxjnl-2020-214642.full AB - Neutrophils play an important role in the lung tumour microenvironment. We hypothesised that radiolabelled neutrophils coupled to single-photon emission CT (SPECT) may non-invasively quantify neutrophil uptake in tumours from patients with non-small cell lung cancer. We demonstrated increased uptake of radiolabelled neutrophils from the blood into tumours compared with non-specific uptake using radiolabelled transferrin. Moreover, indium-111-neutrophil activity in the tumour biopsies also correlated with myeloperoxidase (MPO)-positive neutrophils. Our data support the utility of imaging with In-111-labelled neutrophils and SPECT-CT to quantify neutrophil uptake in lung cancer.