RT Journal Article
SR Electronic
T1 Sputum exosomes: promising biomarkers for idiopathic pulmonary fibrosis
JF Thorax
JO Thorax
FD BMJ Publishing Group Ltd and British Thoracic Society
SP 309
OP 312
DO 10.1136/thoraxjnl-2018-211897
VO 74
IS 3
A1 Makon-Sébastien Njock
A1 Julien Guiot
A1 Monique A Henket
A1 Olivier Nivelles
A1 Marc Thiry
A1 Franck Dequiedt
A1 Jean-Louis Corhay
A1 Renaud E Louis
A1 Ingrid Struman
YR 2019
UL http://thorax.bmj.com/content/74/3/309.abstract
AB Idiopathic pulmonary fibrosis (IPF) is a progressive fibrosing interstitial lung disease of unknown aetiology which leads rapidly to death. As diagnosis of IPF is complex, we aimed to characterise microRNA (miRNA) content of exosomes from sputum of patients with IPF. Using miRNA quantitative PCR array, we found a substantial dysregulation of sputum exosomal miRNA levels between patients with IPF and healthy subjects and identified a unique signature of three miRNAs. Interestingly, we found a negative correlation between miR-142-3p and diffusing capacity of the lungs for carbon monoxide/alveolar volume. This is the first characterisation of miRNA content of sputum-derived exosomes in IPF that identified promising biomarkers for diagnosis and disease severity.