RT Journal Article
SR Electronic
T1 Patients with ARDS show improvement but not normalisation of alveolar surface activity with surfactant treatment: putative role of neutral lipids
JF Thorax
JO Thorax
FD BMJ Publishing Group Ltd and British Thoracic Society
SP 588
OP 594
DO 10.1136/thx.2006.062398
VO 62
IS 7
A1 Philipp Markart
A1 Clemens Ruppert
A1 Malgorzata Wygrecka
A1 Thorsten Colaris
A1 Bhola Dahal
A1 Dieter Walmrath
A1 Heinz Harbach
A1 Jochen Wilhelm
A1 Werner Seeger
A1 Reinhold Schmidt
A1 Andreas Guenther
YR 2007
UL http://thorax.bmj.com/content/62/7/588.abstract
AB Background: Extensive biochemical and biophysical changes of the pulmonary surfactant system occur in the acute respiratory distress syndrome (ARDS). Methods: The effect of intrabronchial administration of a recombinant surfactant protein C-based surfactant preparation (Venticute) on gas exchange, surfactant composition and function was investigated in 31 patients with ARDS in a randomised controlled phase I/II clinical pilot trial. Bronchoalveolar lavage fluids for surfactant analysis were obtained 3 h before and 48 and 120 h after the first surfactant application. Potentially deleterious effects of surfactant neutral lipids in patients with ARDS were also identified. Results: Before treatment all patients had marked abnormalities in the surfactant phospholipid and protein composition. In response to surfactant treatment, gas exchange improved and surfactant phospholipid and protein content were almost normalised. Alveolar surface activity was dramatically impaired before treatment and only partially improved after surfactant administration. Further analysis of the bronchoalveolar lavage fluids revealed a twofold increase in neutral lipid content and altered neutral lipid profile in patients with ARDS compared with healthy controls. These differences persisted even after administration of large amounts of Venticute. Supplementation of Venticute or natural surfactant with a synthetic neutral lipid preparation, mimicking the profile in ARDS, caused a dose-dependent deterioration of surface activity in vitro. Conclusion: Intrabronchial surfactant treatment improves gas exchange in ARDS, but the efficacy may be limited by increased concentration and altered neutral lipid profile in surfactant under these conditions.