PT - JOURNAL ARTICLE AU - K D Lettinga AU - S Weijer AU - P Speelman AU - J M Prins AU - T van der Poll AU - A Verbon TI - Reduced interferon-γ release in patients recovered from Legionnaires' disease AID - 10.1136/thorax.58.1.63 DP - 2003 Jan 01 TA - Thorax PG - 63--67 VI - 58 IP - 1 4099 - http://thorax.bmj.com/content/58/1/63.short 4100 - http://thorax.bmj.com/content/58/1/63.full SO - Thorax2003 Jan 01; 58 AB - Background: Legionella pneumophila, a Gram negative intracellular pathogen, causes Legionnaires' disease (LD). Interferon (IFN)-γ is important for host defence against L pneumophila so reduced IFN-γ production capacity and/or responsiveness might render humans more susceptible to infection with L pneumophila. Methods: Seventy seven patients who suffered from LD after a point source outbreak one year earlier participated in the study. Whole blood was incubated with non-specific stimuli (lipopolysaccharide (LPS) or interleukin (IL)-12) or specific stimuli (viable or heat killed L pneumophila) to evaluate IFN-γ production, and with IFN-γ to evaluate IFN-γ responsiveness. Expression of complement receptor 3 on monocytes was determined by flow cytometry. Thirty seven companions who were also exposed but had not developed LD served as controls. Results: Patients released less IFN-γ than controls in response to stimulation with LPS (mean (SE) 393 (58) pg/ml v 914 (178) pg/ml; p=0.001) and IL-12 (96 (14) pg/ml v 177 (41) pg/ml; p=0.058). IFN-γ responsiveness, measured by release of IFN-γ inducible protein (IP)-10, tumour necrosis factor α, IL-12 production capacity, and monocyte expression of complement receptor 3, did not differ between patients and controls. IFN-γ release after stimulation with LPS and IP-10 release after stimulation with IFN-γ were weakly associated with severity of LD in the former patient group (ρ=–0.3, p=0.011 and ρ=–0.3, p=0.037, respectively). Conclusion: These results suggest that impaired IFN-γ production may contribute to susceptibility to L pneumophila infection.