Article Text
Abstract
Idiopathic pulmonary fibrosis (IPF) is a progressive fibrosing interstitial lung disease of unknown aetiology which leads rapidly to death. As diagnosis of IPF is complex, we aimed to characterise microRNA (miRNA) content of exosomes from sputum of patients with IPF. Using miRNA quantitative PCR array, we found a substantial dysregulation of sputum exosomal miRNA levels between patients with IPF and healthy subjects and identified a unique signature of three miRNAs. Interestingly, we found a negative correlation between miR-142-3p and diffusing capacity of the lungs for carbon monoxide/alveolar volume. This is the first characterisation of miRNA content of sputum-derived exosomes in IPF that identified promising biomarkers for diagnosis and disease severity.
- idiopathic pulmonary fibrosis
- interstitial fibrosis
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Footnotes
M-SN and JG contributed equally.
Contributors MSN and JG designed and performed experiments, analysed the data and wrote the manuscript. MAH collected and processed sputum samples. ON performed experiments. MT performed TEM study. FD, JLC and REL contributed to experiments analysis and interpretation of the results. IS design, analysed, supervised the experiment and wrote the manuscript.
Funding This study was supported by the Fonds d’Investissement de Recherché Scientifique du Centre Hospitalier Universitaire de Liège (FIRS); Centre Hospitalier Universitaire de Liège (CHU); the University of Liège (ULg); the Fonds National de la Recherche Scientifique (FNRS) and the fonds Léon Frédéricq.
Competing interests None declared.
Patient consent Obatined.
Ethics approval The protocol was approved by the ethics committee of CHU of Liège (Belgian number: B707201422832; ref: 2014/302).
Provenance and peer review Not commissioned; externally peer reviewed.
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