This study explored oxidative stress, nicotinamide adenine dinucleotide phosphate oxidase-2 (Nox2) activity and endothelial function in children exposed or not to passive smoking. Compared with controls (n=57), Nox2 activity and isoprostanes were higher in children exposed to passive smoking (n=57); conversely, nitric oxide (NO) bioavailability and flow-mediated dilation were lower in children exposed to passive smoking. A bivariate analysis showed that Nox2 activity correlated with flow-mediated dilation, NO bioavailability and isoprostanes. A multivariate analysis showed that Nox2 activity was significantly associated with serum isoprostanes and cotinine levels; flow-mediated dilation was associated with isoprostanes and carotid intima-media thickness.
In children exposed to passive smoking, Nox2-derived oxidative stress is upregulated and inversely associated with impaired artery dilation.
- passive smoking
- endothelial function
- oxidative stress.
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LL and AMZ contributed equally.
Contributors Study concept and design and guarantors: LL and AMZ. Acquisition of data: FO, LP, GDC, SB and FM. Analysis and interpretation of data: FO, RC, CN, VC and LP. Drafting of the manuscript: LL and AMZ. Critical revision of the manuscript for important intellectual content: FA, MDB, MD and FV. Statistical analysis: LL and FO. Study supervision: FV and MD.
Funding This study was supported by a grant from the University of Rome ‘ La Sapienza’ (Università degli Studi di ROMA ‘ la Sapienza’ RM11715C743A15B7, funds to LL).
Competing interests None declared.
Ethics approval Ethical Committee of ‘Sapienza’ University of Rome (ref. n.2339/26.01.2012).
Provenance and peer review Not commissioned; externally peer reviewed.
Collaborators Francesco Baratta.
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