Article Text

Original article
Rhinovirus-induced interferon production is not deficient in well controlled asthma
  1. Annemarie Sykes1,2,3,4,
  2. Jonathan Macintyre1,2,3,4,
  3. Michael R Edwards1,2,3,
  4. Ajerico del Rosario1,2,3,
  5. Jennifer Haas1,2,3,
  6. Vera Gielen1,2,3,
  7. Onn Min Kon4,
  8. Mark McHale5,
  9. Sebastian L Johnston1,2,3,4
  1. 1National Heart and Lung Institute, Imperial College London, London, UK
  2. 2MRC and Asthma UK Centre in Allergic Mechanisms of Asthma, London, UK
  3. 3Centre for Respiratory Infection, London, UK
  4. 4Imperial College Healthcare NHS Trust, London, UK
  5. 5Respiratory and Inflammation Research Area, AstraZeneca R&D Charnwood, Charnwood, UK
  1. Correspondence to Dr Annemarie Sykes, National Heart and Lung Institute, St Mary's Campus, Imperial College London, London W2 1PG, UK; annemarie.sykes{at}imperial.ac.uk

Abstract

Background Defective rhinovirus (RV)-induced interferon (IFN)-β and IFN-λ production and increased RV replication have been reported in primary human bronchial epithelial cells (HBECs) from subjects with asthma. How universal this defect is in asthma is unknown. Additionally, the IFN subtypes induced by RV infection in primary HBECs have not been comprehensively investigated.

Objective To study RV induction of IFN-α, IFN-β and IFN-λ and RV replication in HBECs from subjects with atopic asthma and healthy controls.

Methods HBECs were obtained from subjects with asthma and healthy controls and infected with RV16 and RV1B, and cells and supernatants harvested at 8, 24 and 48h. IFN proteins were analysed by ELISA and IFN mRNA and viral RNA expression by quantitative PCR. Virus release was assessed in cell supernatants.

Results IFN-β and IFN-λ were the only IFNs induced by RV in HBECs and IFN-λ protein induction was substantially greater than IFN-β. Induction of IFN-λ1 mRNA by RV16 at 48h was significantly greater in HBECs from subjects with asthma; otherwise there were no significant differences between subjects with asthma and controls in RV replication, or in induction of type I or III IFN protein or mRNA.

Conclusions IFN-λ and, to a lesser degree, IFN-β are the major IFN subtypes induced by RV infection of HBECs. Neither defective IFN induction by RV nor increased RV replication was observed in the HBECs from subjects with well controlled asthma reported in this study.

  • Airway Epithelium
  • Asthma
  • Viral infection
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