Background Rates of preterm birth have increased in most industrialised countries but data on later lung function of late preterm births are limited. A study was undertaken to compare lung function at 8–9 and 14–17 years in children born late preterm (33–34 and 35–36 weeks gestation) with children of similar age born at term (≥37 weeks gestation). Children born at 25–32 weeks gestation were also compared with children born at term.
Methods All births from the Avon Longitudinal Study of Parents and Children (n=14 049) who had lung spirometry at 8–9 years of age (n=6705) and/or 14–17 years of age (n=4508) were divided into four gestation groups.
Results At 8–9 years of age, all spirometry measures were lower in the 33–34-week gestation group than in controls born at term but were similar to the spirometry decrements observed in the 25–32-week gestation group. The 35–36-week gestation group and term group had similar values. In the late preterm group, at 14–17 years of age forced expiratory volume in 1 s (FEV1) and forced vital capacity (FVC) were not significantly different from the term group but FEV1/FVC and forced expiratory flow at 25–75% FVC (FEF25–75%) remained significantly lower than term controls. Children requiring mechanical ventilation in infancy at 25–32 and 33–34 weeks gestation had in general lower airway function (FEV1 and FEF25–75) at both ages than those not ventilated in infancy.
Conclusions Children born at 33–34 weeks gestation have significantly lower lung function values at 8–9 years of age, similar to decrements observed in the 25–32-week group, although some improvements were noted by 14–17 years of age.
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What is the key question?
We sought to compare lung function at 8–9 and 14–17 years in children born late preterm (33–34 and 35–36 weeks gestation) with children of similar age born at term (≥37 weeks gestation). We also compared children born at 25–32 weeks gestation with children born at term.
What is the bottom line?
Children born at 33–34 weeks gestation have significantly lower lung function values at 8–9 years of age, similar to decrements observed in the 25–32-week group, although most of these differences were reduced by 14–17 years of age.
Why read on?
The findings from this longitudinal population-based observational cohort study suggest that children born at 33–34 weeks gestation may be at risk of decreased lung function at 8–9 years of age. By 14–17 years of age there were improvements in FEV1.
In most industrialised countries the rate of preterm birth has risen markedly in recent years and late preterm births form a substantial proportion of these. In the USA the rate has increased from 9.5% in 1981 to 12.7% in 2005, with 20% of the preterm births occurring at 32–33 weeks gestation and 60–70% at 34–36 weeks gestation.1 Therefore, even a small increased risk of later respiratory morbidity in infants born beyond 32 weeks gestation will contribute substantially to the number of individuals affected by respiratory disease associated with preterm birth. A workshop convened by the National Institute of Child Health and Human Development recognised the importance of infants born beyond 32 weeks gestation but identified the lack of outcome data for this population.2 It is, however, increasingly recognised that late preterm infants have increased respiratory morbidity in the neonatal period, especially neonatal respiratory distress syndrome, transient tachypnoea of the newborn and pneumonia.3 Few studies have reported later respiratory outcomes in children born late preterm,4 5 with most studies on lung function outcomes following preterm birth focusing on extremely preterm infants of 32 weeks gestation or less, especially as they are at risk of developing chronic lung disease of prematurity (CLD).6
Prematurity is associated with delivery of an infant at early stages of lung development with the focus traditionally having been on those born at 32 weeks gestation or less.7 As these infants are born at the late canalicular and early saccular stages of lung development, they often require intervention especially to support their respiratory system. However, those born ‘late’ preterm (ie, 33–34 weeks gestation) are also at a vulnerable stage of lung development (the late saccular stage),8 and it is unknown if normal lung growth and development continues normally in the extrauterine environment after preterm birth. Infants born at 35–36 weeks gestation are often termed ‘near term’ infants (eg, in the ECMO and Nitric Oxide clinical trials9–11) and are often assumed to behave like term infants, but it is unclear if being born at an early stage of alveolar development also results in adverse longer term respiratory outcomes.
In this study we sought to compare lung function at 8–9 and 14–17 years in a population-based cohort of children born late preterm at 33–34 weeks gestation and near term at 35–36 weeks gestation with children of similar age born at term (≥37 weeks gestation). We also compared the results for these two gestation groups with children of similar age born extremely preterm at 25–32 weeks gestation. The longitudinal change in lung function between 8–9 and 14–17 years of age is also reported.
Avon Longitudinal Study of Parents and Children (ALSPAC)
The study used data from the Avon Longitudinal Study of Parents and Children (ALSPAC) which has been described previously.12 Additional detail is provided in the online supplement.
Gestation groups and perinatal data
Gestational age was based on maternal reporting and medical assessments.13 All children who had spirometry results at either 8–9 or 14–17 years of age were divided into four groups based on gestational age: 25–32 weeks, 33–34 weeks, 35–36 weeks and ≥37–43 weeks (term control). Perinatal data on respiratory management were extracted from hospital records. Additional detail is provided in the online supplement.
Self-reported respiratory symptoms and tobacco smoke exposure
As part of ALSPAC studies, questionnaires at 7.6 years of age established if children ‘ever had asthma diagnosed by a doctor together with symptoms and/or treatment in the previous 12 months’. Questionnaires at 13.8 years of age established if the children ‘ever had asthma diagnosed by a doctor’. Regular smoking at age 14 was defined as the usual smoking of at least one cigarette per day or at least six cigarettes per week.
Spirometry was performed at 8–9 and 14–17 years of age as previously reported.14 The variables forced expiratory volume in 1 s (FEV1), forced vital capacity (FVC) and forced expiratory flow at 25–75% of FVC (FEF25–75) were recorded and transformed to SD scores adjusted for age, sex and height according to the method of Chinn et al in the ALSPAC cohort independently at 8–9 and 14–17 years of age.15 FEV1/FVC and FEF25–75/FVC were calculated as markers of airway obstruction and dysanapsis.16
At 8–9 years of age children, unselected for asthma or wheezing, had a bronchial challenge test with methacholine using the method of Yan et al,17 as previously described.14 Additional detail is given in the online supplement.
Gestations of ≥44 weeks were considered implausible and thus excluded. The birth weight z-scores were calculated using the lms Growth program (Medical Research Council, UK) to take into account gestation, as the numbers in the earlier gestation groups were small and the birth weights of the ALSPAC cohort were similar to published reference values (data not shown). Weight at the time of lung function testing was converted into a z-score within the ALSPAC cohort as the ALSPAC data showed a trend towards earlier growth (data not shown). Spirometry values are summarised using the mean with 95% CIs. For longitudinal changes in spirometry, only data from children who had spirometry at both time points were included in the analyses. To account for confounders the spirometry data, already adjusted for age, gender and height, were further adjusted using general linear modelling for smoking during pregnancy and social class status based on father's occupation, which are well-described confounders of lung function and preterm delivery. Missing values of each confounder were coded into a separate missing category. Adjusted scores were calculated and used in subsequent analyses. Longitudinal changes were assessed using paired t tests on the adjusted spirometry values. Differences between different gestational age groups and the term group and between ventilated and non-ventilated infants were tested using independent samples t tests. The results for ventilated and non-ventilated infants are given unadjusted for social class and smoking during pregnancy but are adjusted for age, gender and height. Bronchial hyper-responsiveness was compared between gestation groups by one-way analysis of variance. χ2 Tests were used to compare asthma rates between gestation groups at 7.6 and 13.8 years of age. p values <0.05 were considered statistically significant. All analyses were performed using SPSS V.16 (SPSS Inc).
From 14 049 live births, spirometry data were available for 6705 (47.7%) children at 8–9 years of age and 4508 (32.1%) at 14–17 years of age, with measurements at both ages available for 3799 (27.0%) children (figure 1). Tables 1 and 2 show the characteristics of the children in each gestation group. A higher percentage of children in the 25–32-week gestation group were from lower social classes and had a higher prevalence of maternal smoking. A higher percentage of children who did not attend for spirometry were from a lower social class and had higher exposure to maternal smoking (table 3). 76% of the extremely preterm group and 17% of the late preterm group required mechanical ventilation but few (≤1%) did so in the more mature groups (table 2).
Asthma in the previous year at 7.6 years of age was reported by more than 20% in the 25–32-week and 33–34-week gestation groups but by <15% in the 35–36-week gestation and term groups, although these differences were not statistically significant. The rates of asthma ever at 13.8 years of age were similar (23–30%) in all groups. No differences were noted for smoking at 14 years of age between the gestational groups. Of the children who had any lung spirometry, 104 children reported regular smoking at 14 years of age (3 in the 35–36-week gestation group, 101 in the term group and none in those born at <35 weeks gestation).
Association of preterm birth with lung function in later childhood
Spirometry data by gestation group at 8–9 and 14–17 years of age are shown in tables 4 and 5, respectively. At 8–9 years of age, all spirometry measures were significantly lower in the 33–34-week gestation group compared with the term group whereas values in the 35–36-week gestation group were similar to term infants. In the 25–32-week gestation group, all spirometry measures except FVC were significantly lower compared with the term group. The decrements of lung function were similar (table 4) between the 25–32-week and the 33–34-week gestation groups. The approximate differences compared with term infants after adjustment for age, gender and height were −143 ml for FEV1, −98 ml for FVC and −253 ml/s for FEF25–75 in the 33–34-week gestation group and −121 ml for FEV1, −65 ml for FVC and −297 ml/s for FEF25–75 in the 25–32-week gestation group.
At 14–17 years of age, FEF25–75, FEF25–75/FVC and FEV1/FVC remained significantly lower in the late preterm group than in the term group but FEV1 and FVC were not significantly different. The approximate differences from those born at term after adjustment for age, gender and height were −20 ml for FEV1, 156 ml for FVC and −382 ml/s for FEF25–75, in the 33–34-week gestation group and −114 ml for FEV1, −120 ml for FVC and −428 ml/s for FEF25–75 in the 25–32-week gestation group.
Changes in spirometry between 8–9 years and 14–17 years of age
Table 6 shows changes in spirometry for children with measurements at both time points. For the 33–34-week gestation group, significant increases were observed for FEV1. In contrast, no significant changes were noted between the time periods for the 25–32-week, 35–36-week and term groups.
Effect of ventilation in the newborn period on later lung function
Table E1 (in the online supplement) shows spirometry for the extremely and late preterm children according to ventilation requirements in the newborn period. In general, children who were ventilated in infancy had lower airway function (FEV1 and FEF25–75) at both ages compared with those not ventilated in infancy. More results are shown in the online supplement.
No significant differences were found between the different gestation groups for the geometric mean provocative dose of methacholine causing a fall in FEV1 of 20% or more (PD20) in either responders or the whole study population (table 7).
This study shows that most lung function variables measured by spirometry at 8–9 years of age were significantly lower in infants born at 33–34 weeks gestation compared with term infants, with the effect sizes being very similar to those in the 25–32-week gestation group. By 14–17 years of age the differences in FEV1 and FVC attenuated but differences with term controls persisted for FEF25–75%, FEV1/FVC and FEF25–75%/FVC in the 33–34-week gestation group. In contrast, children born at 35–36 weeks had comparable lung function with children born at term at both follow-ups. While the numbers were small, the indications were that those in the 33–34-week gestation group who required mechanical ventilation fared worse than those who did not.
Late preterm infants of 33–34 weeks gestation are a growing population which is increasingly recognised to be at risk of morbidity in the neonatal period from respiratory diseases including respiratory distress syndrome,18 and in infancy from respiratory viruses such as respiratory syncytial virus.19 A large study of over 150 000 singleton births at 34–41 weeks gestation showed rates of severe respiratory disorders in neonates decreased with increasing gestational age (from 19.8% at 34 weeks to 0.28% at 39–41 weeks).20 Using the raised volume rapid thoracic compression technique, Friedrich et al reported decreased airway flows despite normal FVC at 4 and 16 months of age in healthy late preterm infants. However, it is unclear if decreased lung function persists into childhood and beyond. Our data show that the preterm group of 33–34 weeks gestation have lower spirometry measurements than those born at term at 8–9 years of age; however, improvement was observed for FEV1 by 14–17 years of age but not for other measurements. The extremely preterm group had similar effect sizes but, in contrast, the 35–36-week gestation group had similar lung function to the term control group at both ages. Infants at 33–34 weeks gestation are born at the stage of saccular development8 and early delivery may alter their subsequent alveolar development, as has been suggested for extremely preterm infants.21 Interventions such as mechanical ventilation and oxygen therapy are well known to promote pulmonary inflammation in infancy leading to abnormal growth and development.6 Although the numbers are small, especially as only 17% of the 33–34-week gestation group required mechanical ventilation in infancy, the data appear to support the notion that intervention is associated with worse spirometry at 8–9 years, which did not appear to improve to the same degree as for the non-ventilated group between the two ages (table E1 in online supplement). The interpretation is further complicated by the fact that mechanical ventilation was likely to be instituted in infants with underlying lung disease which may already identify them as candidates for future lung abnormalities. Other possible explanations for the poorer lung function in the 33–34-week gestation group are an adverse prenatal environment associated with preterm delivery (eg, chorioamnionitis may affect later lung function)22–25 or dysregulated lung growth associated with premature cessation of late gestational development. The loss of late intrauterine lung maturation in the 33–34-week gestational group suggests that lung growth may be dysregulated after birth resulting in physiological differences compared with those completing term gestation.
We noted poorer lung function in childhood in the late preterm children which was similar to the extremely preterm group, but did not note significant differences in rates of asthma between the gestation groups. It would be interesting to investigate if the decreased FEV1 can be reversed by bronchodilators as was recently shown for children who were born extremely preterm.26 Furthermore, if there is plasticity of the airways, bronchodilators may be effective in this group of children, but whether they improve longer term airway function needs further investigation. Few studies have reported longitudinal lung function in preterm infants with most focusing on severe long-term respiratory consequences—that is, those who develop CLD. In a small number of children with CLD, often also called bronchopulmonary dysplasia, Filippone et al reported that airflow limitation tracked between 2 and 8.8 years of age did not show improvements between the two time points.27 In contrast, Doyle and colleagues reported significant improvements in predicted values of lung function between 8 and 14 years of age in children with a birth weight <1501 g.28 When we compared the preterm children who had spirometry at both time periods, our data support potential improvements in lung function in children born at 33–34 weeks gestation from 7–8 years of age to 14–17 years, especially for the larger airways. For the 25–32-week gestation group, relative improvements were noted in the z-scores for lung spirometry but these were not statistically significant. While tracking appears to be an attractive concept, our longitudinal data question the concept of tracking as lung function improved between 7–8 and 14–17 years of age. More recent imaging modalities such as the use of hyperpolarised helium may assist in investigating lung growth and development beyond infancy29 to clarify if, indeed, continued growth and development occurs beyond infancy.
While we acknowledge that the data were not specifically collected to address the current hypothesis, the strength of this study is that it uses data from a large unselected population-based cohort with comprehensive longitudinal data with reasonable numbers of preterm-born children and a large number in the term control group. A further strength is that spirometry was measured using the same methods at the two time points and is an objective outcome. As with all cohort studies, a major weakness is the loss to follow-up and the resulting potential attrition bias. Fewer children who had mothers who smoked during pregnancy and a from lower social class attended for lung spirometry compared with the attenders. Since lower social class is associated with more preterm births, more maternal smoking and decreased attendance for spirometry, it is possible that a higher rate of attendance in these children would have increased the observed differences. In addition, ALSPAC did not measure the lung function of severely disabled children and, as these represent the severe end of morbidity associated with preterm delivery, the differences may have been attenuated by their exclusion. We included children from ethnic minorities in our analysis as there were very few of them (in the whole ALSPAC cohort only 5% of the children who reported their ethnic background reported being non-white), and the ALSPAC cohort is broadly representative of the population of the UK.12
In summary, our data show that, at 8–9 years of age, measures of airway function are reduced in children born at 33–34 weeks gestation compared with children born at term, and are of similar magnitude to those in the extremely preterm group who received a much higher proportion of respiratory intervention during the neonatal period. However, by 14–17 years of age, there was no significant difference in FEV1 and FVC in the late preterm group compared with children born at term, although FEV1/FVC and FEF25–75% remained significantly lower than term controls. This raises the question about what occurs from a lung development point of view between 33–34 and 35–36 weeks of gestation. Now that there is a strong suspicion of respiratory physiological abnormalities in late preterm-born children, it will be interesting to study in more detail whether they are at greater risk of respiratory infections in infancy, have increased respiratory symptoms or indeed have limitation of exercise with or without exercise-induced bronchoconstriction.
We are grateful to all the families who took part in this study, the midwives for their help in recruiting them and the whole ALSPAC team which includes interviewers, computer and laboratory technicians, clerical workers, research scientists, volunteers, managers, receptionists and nurses. We thank Dr David Herrick for his help in identifying relevant perinatal data.
Review history and Supplementary material
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Funding The Avon Longitudinal Study of Parents and Children (ALSPAC) receives core funding from the UK Medical Research Council, the Wellcome Trust and the University of Bristol. The lung function measures were supported by a grant from the UK Medical Research Council (G0401540). SJK was partially supported by Nutricia Research Foundation.
Competing interests None.
Ethics approval This study was conducted with the approval of the ALSPAC Law and Ethics Committee and the local research ethics committees.
Provenance and peer review Not commissioned; externally peer reviewed.
Data Sharing Statement Data generated by the ALSPAC study are held as a national resource. Data are anonymised at entry and held separately from administrative data; therefore, identification of individual subjects' data is concealed from researchers. Access to data items is provided on application to the ALSPAC Executive Committee. The full ALSPAC collaboration policy, including details of data sharing arrangements, is published online at: http://www.bris.ac.uk/alspac/sci-com/collab-policy/.
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