Article Text

Original article
Clinical and laboratory features distinguishing pandemic H1N1 influenza-related pneumonia from interpandemic community-acquired pneumonia in adults
  1. Thomas Bewick1,
  2. Puja Myles2,
  3. Sonia Greenwood1,
  4. Jonathan S Nguyen-Van-Tam2,
  5. Stephen J Brett3,
  6. Malcolm G Semple4,
  7. Peter J Openshaw5,
  8. Barbara Bannister6,
  9. Robert C Read7,
  10. Bruce L Taylor8,
  11. Jim McMenamin9,
  12. Joanne E Enstone2,
  13. Karl G Nicholson10,
  14. Wei Shen Lim1,
  15. Influenza Clinical Information Network (FLU-CIN)
  1. 1Department of Respiratory Medicine, Nottingham University Hospitals NHS Trust, Nottingham, UK
  2. 2Division of Epidemiology and Public Health, University of Nottingham, Nottingham, UK
  3. 3Centre for Peri-operative Medicine and Critical Care Research, Imperial College Healthcare NHS Trust, London, UK
  4. 4Department of Women's and Children's Health, University of Liverpool, Liverpool, UK
  5. 5Centre for Respiratory Infections, National Heart and Lung Institute, Imperial College, London, UK
  6. 6Department of Health, Skipton House, London, UK
  7. 7Department of Infection and Immunity, University of Sheffield, Royal Hallamshire Hospital, Sheffield, UK
  8. 8Department of Critical Care, Portsmouth Hospitals NHS Trust, Portsmouth, UK
  9. 9Health Protection Scotland, NHS National Services, Glasgow, UK
  10. 10Infectious Diseases Unit, University Hospitals of Leicester NHS Trust, Leicester Royal Infirmary, Leicester, UK
  1. Correspondence to Dr Wei Shen Lim, Respiratory Medicine, Nottingham University Hospitals NHS Trust, City Hospital Campus, Hucknall Road, Nottingham NG5 1PB, UK; weishen.lim{at}


Background Early identification of patients with H1N1 influenza-related pneumonia is desirable for the early instigation of antiviral agents. A study was undertaken to investigate whether adults admitted to hospital with H1N1 influenza-related pneumonia could be distinguished clinically from patients with non-H1N1 community-acquired pneumonia (CAP).

Methods Between May 2009 and January 2010, clinical and epidemiological data of patients with confirmed H1N1 influenza infection admitted to 75 hospitals in the UK were collected by the Influenza Clinical Information Network (FLU-CIN). Adults with H1N1 influenza-related pneumonia were identified and compared with a prospective study cohort of adults with CAP hospitalised between September 2008 and June 2010, excluding those admitted during the period of the pandemic.

Results Of 1046 adults with confirmed H1N1 influenza infection in the FLU-CIN cohort, 254 (25%) had H1N1 influenza-related pneumonia on admission to hospital. In-hospital mortality of these patients was 11.4% compared with 14.0% in patients with inter-pandemic CAP (n=648). A multivariate logistic regression model was generated by assigning one point for each of five clinical criteria: age ≤65 years, mental orientation, temperature ≥38°C, leucocyte count ≤12×109/l and bilateral radiographic consolidation. A score of 4 or 5 predicted H1N1 influenza-related pneumonia with a positive likelihood ratio of 9.0. A score of 0 or 1 had a positive likelihood ratio of 75.7 for excluding it.

Conclusion There are substantial clinical differences between H1N1 influenza-related pneumonia and inter-pandemic CAP. A model based on five simple clinical criteria enables the early identification of adults admitted with H1N1 influenza-related pneumonia.

  • Pneumonia
  • influenza
  • H1N1
  • pandemic
  • respiratory infection
  • bacterial infection
  • viral infection

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In March 2009 the first cases of a novel strain of influenza A virus of swine origin were reported in Mexico1 and, within 3 months, global spread led to declaration of a pandemic by the World Health Organization (WHO). While most cases of pandemic influenza H1N1 infection have been mild or subclinical,2–4 some patients experienced severe illness from H1N1 influenza infection and others severe influenza-related complications.5 6

Pneumonia is one of the commonest and most important complications of influenza infection. Influenza virus causes primary viral pneumonia, and secondary bacterial infections are also recognised.7 8 In contemporary cohorts of patients admitted with inter-pandemic community acquired pneumonia (CAP), influenza is frequently found as a co-pathogen alongside other respiratory pathogens such as Streptococcus pneumoniae.9–12 Studies of the 1918–19 influenza pandemic have suggested that the majority of influenza-related deaths during that period were caused by secondary bacterial pneumonia.13 More recently, in hospitalised patients with confirmed H1N1 influenza infection, radiological evidence of pneumonia was observed in 18–66% of patients.14–17 In addition, although not necessarily the cause of death, pneumonia was found in the majority of fatal cases at post-mortem examination.18 19 Overall, fewer than 30% of H1N1 influenza-related pneumonia cases have evidence of bacterial co-infection,20–22 suggesting that primary viral pneumonia is often important.

Early identification of patients with H1N1 influenza-related pneumonia may enable the early administration of antiviral agents with possible improved outcomes.23 However, there are few data relating to the clinical differentiation of H1N1 influenza-related pneumonia from inter-pandemic CAP. The aims of the current study were (1) to compare and contrast the clinical features of adult patients admitted with CAP versus H1N1 influenza-related pneumonia and (2) to develop a model that identifies H1N1 influenza-related pneumonia using simple clinical criteria.


Study patients

H1N1 influenza-related pneumonia cohort (H1N1 cohort)

Between May 2009 and January 2010, the Influenza Clinical Information Network (FLU-CIN) collected clinical and epidemiological data on patients admitted to UK hospitals with confirmed H1N1 influenza infection. Seventy-five hospitals in 31 cities or towns were included. The details of data collection and the overall findings from the first wave of the 2009 pandemic have been described elsewhere.24 H1N1 influenza infection was diagnosed by a positive polymerase chain reaction (PCR) result from respiratory samples obtained via a nasopharyngeal swab or bronchoalveolar lavage performed during the admission episode. Data collected included demography, clinical observations, clinical course, laboratory and radiological test results and outcome. The current study cohort comprised adults (aged ≥16 years) whose admission chest x-rays met one of the following criteria:

  1. Chest x-ray report clearly suggestive of pneumonia.25

  2. Chest x-ray report showed acute infiltrates but no consolidation.

  3. No chest x-ray report available but x-ray documented in the clinical notes as being in keeping with pneumonia (n=24).

Patients who had acquired H1N1 influenza infection while in hospital or had been transferred to a study site from another hospital (eg, for extracorporeal membrane oxygenation therapy) were excluded.

Non-H1N1 influenza CAP cohort (CAP cohort)

Between September 2008 and June 2010, consecutive adult patients (aged ≥16 years) admitted to a large UK teaching hospital trust (Nottingham University Hospitals NHS trust) with CAP were prospectively recruited as part of a population-based observational cohort study. Patients were included if they had at least one acute symptom in keeping with a lower respiratory tract infection (breathlessness, cough, sputum production or fever), had new infiltrates on a chest x-ray and were treated by the admitting team for CAP. Patients were excluded if they had been admitted to hospital in the preceding 10 days, had tuberculosis, or had post-obstructive pneumonia due to lung cancer. Participants were identified by study investigators on a daily basis from the acute admitting medical wards and enrolled following informed consent. All patients were managed in a similar manner according to trust CAP guidelines at the discretion of the attending clinician. For the purposes of this analysis and to ensure inclusion of only cases without H1N1 influenza infection, participants were excluded if admitted during the period of H1N1 influenza circulation in Nottingham (between 30 April 2009 and 10 February 2010—this interval comprising all cases of H1N1 influenza infection in the Nottingham area based on local Health Protection Agency data (unpublished)).

Statistical methods

Data were analysed using SPSS Version 16.0. Continuously distributed variables were compared between H1N1 and CAP cohorts using the Student's t test if data were normally distributed and the Mann–Whitney U test if non-normally distributed. Categorical data were compared using Pearson χ2. In order to derive a clinical diagnostic model for H1N1 influenza-related pneumonia, continuously distributed variables were re-categorised into binary variables based on thresholds derived from established acute severity scores (CURB-65 score,26 Pneumonia Severity Index,27 Surviving Sepsis Campaign28) and univariate analysis using χ2 allowed calculation of odds ratios with 95% confidence intervals. Variables for inclusion in the final model were selected using automatic stepwise regression with both forwards (selection) and backwards (deletion) variants. The efficacy of the model for predicting H1N1 influenza-related pneumonia was then assessed by calculating the area under the curve (AUC) of the receiver-operating characteristic (ROC) curve.


Patient characteristics

Of 1046 adults with confirmed H1N1 influenza infection in the FLU-CIN cohort, 266 (25.4%) had evidence of either radiographic consolidation or other infiltrates consistent with acute infection. Twelve patients were transferred in from hospitals outside the study area or developed influenza infection while already an inpatient, leaving a study cohort of 254 patients (H1N1 cohort). The comparator group comprised 648 patients with inter-pandemic CAP (CAP cohort). The patient characteristics are summarised in table 1 and the relative age distribution of both cohorts is shown in figure 1. Despite having similar in-hospital mortality (H1N1 cohort 11.4%; CAP cohort 14.0%), the two groups differed substantially. The median age of patients in the H1N1 cohort was 42 years compared with 75 years in the CAP cohort (p<0.001). The most common comorbid illnesses in the H1N1 cohort were asthma (25.2%) and diabetes mellitus (9.8%) compared with chronic obstructive pulmonary disease (COPD) (26.1%) and diabetes mellitus (15.4%) in the CAP cohort. In the H1N1 cohort, 9.4% of patients had ≥3 comorbid illnesses compared with 11.9% of controls. In addition, patients in the H1N1 cohort were more likely to be febrile, tachycardic, have bilateral radiographic abnormalities and have lower leucocyte counts and levels of C-reactive protein. Confusion, comorbidity and blood urea levels were higher among patients in the CAP cohort. Within the H1N1 cohort, 11 (4.3%) were pregnant and 21 (8.3%) were obese. The value of the CURB-65 score in predicting inpatient mortality was assessed by calculating the AUC for ROC curves for each group; the AUC for the H1N1 cohort was 0.650 compared with 0.741 for the CAP cohort.

Table 1

Clinical characteristics of patients with H1N1 influenza-related pneumonia compared with community-acquired pneumonia (CAP)

Figure 1

Age distribution of patients with H1N1 influenza-related pneumonia (group A) compared with community-acquired pneumonia (group B).

Derivation of a clinical model for the diagnosis of H1N1 influenza-related pneumonia

Nine categorical variables were found to be associated with H1N1 influenza-related pneumonia on univariate analysis (table 2). Two of these variables (C-reactive protein and albumin) were excluded from the derivation of the diagnostic prediction model because they are not routinely performed on admission in all hospitals for acutely ill patients. In a logistic regression model, five of the seven variables were found to be statistically significant at the 5% level (table 3). These variables remained statistically significant using both forwards and backwards stepwise regression models. The resulting 5-point score generated a ROC curve AUC of 0.873 (based on 858 data points). The derived score stratifies patients into three risk groups for H1N1 influenza-related pneumonia as shown in table 4. In order to assess the impact of age on the discriminant model, a further analysis was undertaken with age excluded from the logistic regression analysis, leaving the four variables of bilateral radiographic consolidation, mental orientation, leucocyte count ≤12×109/l and temperature ≥38°C. The resulting ORs were 3.0 (95% CI 2.0 to 4.4), 6.3 (95% CI 3.5 to 11.5), 9.4 (95% CI 6.3 to 14.3) and 2.6 (95% CI 1.8 to 3.8), respectively. The ROC curve AUC for this model was 0.806. Use of age ≤65 years as a single binary predictor gave an AUC of 0.794.

Table 2

Univariate association between selected clinical characteristics and presence of H1N1 infection

Table 3

Multivariate analysis of variables associated with H1N1 influenza-related pneumonia

Table 4

Proportions of patients identified with H1N1 influenza-related pneumonia according to the diagnostic prediction model

C-reactive protein and albumin were excluded from the initial logistic regression analysis in order to maximise clinical utility of the algorithm. When both variables were included as part of a multivariate analysis for exploratory purposes (H1N1 cohort, n=123; CAP cohort, n=456), only albumin ≤30 g/dl was statistically significant at the 5% level (OR 1.9; 95% CI 1.1 to 3.5). The five previously described variables of age ≤65 years, bilateral radiographic consolidation, mental orientation, leucocyte count ≤12×109/l and temperature ≥38°C remained statistically significant within the model.

As the age profiles of the two cohorts were significantly different and age may be a surrogate for other confounding factors, a secondary analysis was undertaken including only those patients aged <65 years (H1N1 cohort, n=228; CAP cohort, n=200). Within this subgroup, median age remained significantly different (39 years vs 50 years; p<0.001). In a logistic regression analysis, leucocyte count ≤12×109/l (OR 10.1, 95% CI 6.1 to 16.7), age ≤50 years (OR 2.7, 95% CI 1.6 to 4.4), bilateral radiographic change (OR 2.5, 95% CI 1.5 to 4.4) and temperature ≥38°C (OR 2.1, 95% CI 1.3 to 3.4) were the only significant contributors to the final model. Using this model to predict H1N1 influenza-related pneumonia, the AUC for the ROC curve was 0.789.

A model to distinguish H1N1 influenza-related pneumonia based solely on physiological and radiological variables available rapidly in the emergency department (ie, excluding laboratory results) is shown in the online supplement.


To our knowledge, this is the first study to highlight the clinical differences between inter-pandemic CAP and H1N1 influenza-related pneumonia. Patients with H1N1 influenza-related pneumonia were significantly younger than patients with CAP, reflecting the epidemiology of H1N1 influenza infection globally.2 14 24 29 30 This may relate to previous exposure of older persons to pre-2009 H1N1 influenza viruses conferring some immunity.31–33 In future years, as the virus evolves, the epidemiology of H1N1 infection may alter to affect older persons to a greater extent. Such a shift would also impact on the age distribution of patients developing H1N1 influenza-related pneumonia with potentially important consequences on resultant morbidity and mortality. Studies of CAP conducted in inter-pandemic years demonstrate an association between extremes of age or frailty and influenza or viral infection.10 11

Lower levels of C-reactive protein and leucocytes were observed in patients with H1N1 influenza-related pneumonia. This finding is consistent with reports from studies of inter-pandemic influenza-related pneumonia of relatively low leucocyte, neutrophil and C-reactive protein levels.10–12 These markers of inflammation are driven mainly by bacterial infections, and the predominance of primary viral pneumonia as opposed to secondary bacterial pneumonia in H1N1 influenza-related pneumonia may therefore partially account for this observation.16 34 Despite the lower levels of C-reactive protein and leucocytes, patients with H1N1 influenza-related pneumonia had higher levels of fever than patients with CAP. This may be related to high levels of production of proinflammatory cytokines, particularly interleukin 6,35 with reduced innate and adaptive responses to S pneumoniae seen with pandemic H1N1 influenza.36 It may also partially reflect the relative inability of older subjects to mount a febrile response to serious infection.37

A diagnostic prediction model was derived from five simple clinical criteria—age ≤65 years, presence of bilateral radiographic consolidation, absence of confusion as measured by orientation in time, place or person, leucocyte count ≤12×109/l and temperature ≥38°C. Assigning one point for each clinical criterion present, a score of 4 or 5 gave a positive likelihood ratio of 9.0 for predicting H1N1 influenza-related pneumonia, while a score of 0 or 1 gave a positive likelihood ratio of 75.7 for excluding H1N1 influenza-related pneumonia. Although age is by far the single strongest predictor (OR 19.6), its predictive value is inferior to the proposed model comprising five variables. When age was removed from the proposed model, the remaining four variables—bilateral radiographic consolidation, mental orientation, leucocyte count ≤12×109/l and temperature ≥38°C—retained their significance as independent predictors of H1N1 influenza-related pneumonia, attesting to their importance within the model. This model is applicable at the time of admission when a diagnosis of CAP is determined and would be useful in aiding the early diagnosis of patients with H1N1 influenza-related pneumonia as well as management decisions relating to infection control and the instigation of early empirical antiviral therapy. Although the efficacy of antiviral agents in the treatment of patients hospitalised with influenza-related pneumonia is not scientifically proven,38 evidence from observational studies in hospital settings suggests that early antiviral use does improve clinical outcomes.14 23 39 40

Currently, this diagnostic prediction model can only be advocated for H1N1 influenza-related pneumonia. It should not be applied to other viral pneumonias without further validation. Similarly, should pandemic influenza A/H1N1 2009 evolve and affect older persons more widely, the predictive value of the model is likely to be altered.

Disease severity assessment is crucial in the management of patients with pneumonia. The CURB-65 severity assessment score has been validated for use in predicting 30-day mortality in patients with CAP26 and was advocated as potentially useful in patients with influenza-related pneumonia.41 42 However, this study suggests that, in patients with H1N1 influenza-related pneumonia, the CURB-65 score does not perform so well. This difference may be due to a combination of features including the lower age distribution and comorbidity profile of patients with H1N1 influenza-related pneumonia and differences in the inflammatory response to H1N1 influenza infection.43 Further study of the prognostic factors relevant to H1N1 influenza-related pneumonia is warranted.

Study limitations

The main limitation to this study is that the CAP cohort was not collected contemporaneously with the H1N1 cohort and, while the latter patients were distributed nationally, patients in the CAP cohort were recruited from a single study centre. Patients in the CAP cohort were prospectively enrolled as part of a population-based cohort study involving all patients hospitalised with CAP in the Nottingham area (catchment population 750 000). Nottingham has a record of conducting large cohort studies of CAP.44–47 The age and demographics of this CAP study cohort mirror the demographics of patients with CAP described in a recently completed national multicentre audit of CAP organised by the British Thoracic Society (unpublished data). These factors support the view that the CAP cohort is representative of patients admitted to hospital with CAP.

Patients recruited to the FLU-CIN cohort were identified following testing for H1N1 influenza on the basis of clinical suspicion rather than rigorously applied testing criteria. Nevertheless, the FLU-CIN cohort is similar to other large published H1N1 influenza cohorts in terms of clinical features and rates of pneumonia (22–37%) and critical care admission (13–25%).14 48 49 17 These observations suggest that the FLU-CIN cohort is indeed representative of patients hospitalised with H1N1 infection in the UK and is not biased in relation to patients with influenza-related pneumonia or patients with severe disease.

All patients with H1N1 influenza-related pneumonia had laboratory-confirmed pandemic H1N1 influenza infection so misclassification is eliminated. In contrast, it remains possible that the CAP cohort may have contained patients in whom the precipitating cause of pneumonia was influenza, despite the exclusion of patients admitted with CAP during the period when pandemic H1N1 influenza was circulating in the study catchment population. The inclusion of some individuals with influenza-related pneumonia within the CAP cohort would tend towards increasing the similarities between the two groups thereby producing underestimates of any differences.


H1N1 influenza-related pneumonia differs substantially from inter-pandemic CAP. A diagnostic prediction model based on the five clinical features of age (≤65 years), leucocyte count (≤12×109/l), bilateral radiographic change, temperature (≥38°C) and mental orientation (time/person/place) allows the early discrimination of H1N1 influenza-related pneumonia from CAP following hospital admission and confers confidence to the instigation of early empirical antiviral therapy.


The authors thank Elaine Gadd and Colin Armstrong, Department of Health, England for their invaluable contribution in relation to the FLU-CIN programme and also gratefully acknowledge individuals who helped identify cases and collated clinical data: Alison Booth, Margaret Charlesworth, Sarah Rodenhurst, Angela Ballard and Alison Holmes at Imperial College Healthcare NHS Trust, London, UK; Sally Batham, Phayre Parkinson, Tracy Kumar and Aiden Dunphy at the University Hospitals of Leicester NHS Trust, Leicester, UK; Anne Tunbridge, Patty Hempsall, Joyce Linskill, Aimee Turner, Sharon Grindle, Dawn Shelvin and Eric Moulds at Sheffield University Hospitals NHS Trust, Sheffield, UK; Elvina White, Elaine Scott, Jennifer Cater, Erica Sergi and Helen Hill at Alder Hey Children's Hospital NHS Foundation Trust, Liverpool, UK; Deborah Fleetwood, Lorna Roche, Sarah Dyas and Maria Boswell at the Royal Liverpool and Broadgreen University Hospital's Trust, Liverpool, UK; Gillian Vernon, Gillian Houghton, Heather Longworth and Angela Kerrigan at Liverpool Women's Hospital, Liverpool, UK; Gemma Thompson, Emily Jarvis and Charlotte Minter at the Nottingham University Hospitals NHS Trust, Nottingham, UK; Kristina Lum Kin, Jacqueline Daglish, Sam Hayton and Gemma Slinn at Birmingham Children's Hospital, Birmingham, UK; Michelle Lacey and Ali McAllister at Quality Improvement Scotland; Karen Duffy, Anne Gordon and Kevin Rooney at Greater Glasgow and Clyde NHS Hospitals, colleagues in Scotland who contributed to the FLU-CIN data - Eleanor Anderson, Malcolm McWhirter, Hilary Davison, William Carmen, Alastair MacConnachie, Mark Cotton, Arlene Reynolds, Heather Murdoch, Karen Voy and Rosie Hague; Brian Smyth and Cathriona Kearns from the National Public Health Agency, Northern Ireland for identifying cases and facilitating data collection; Teresa Cunningham at the Southern Trust and Leslie Boydell at the Belfast Trust for facilitating data collection; Alemayehu Amberbir, Safaa Al-Badri, Baraa Mahgoob and Nachi Arunachalam at the University of Nottingham for data entry and obtaining background population data; also Graham Watson for database development and support. The authors also thank Sir Gordon Duff, Co-Chair of the Scientific Advisory Group for Emergencies, and Janet Darbyshire, Co-Chair of the Influenza Clinical Information Network Strategy Group, for their support; Shona Kelly of the University of Nottingham for help in developing the initial FLU-CIN data collection tool; and Patrick O'Brien of University College London Hospitals NHS Foundation Trust for assistance in further developing the data set. They thank those Chief Executive Officers, clinicians, virologists and managers too numerous to mention who were active in notifying cases to FLU-CIN. SJB and PJMO wish to acknowledge the support of the UK NIHR Biomedical Research Centre scheme.


Supplementary materials

  • Web Only Data thx.2010.151522

    Files in this Data Supplement:


  • Linked article 157404.

  • Funding The Department of Health in England was the main funder for FLU-CIN. Chairmanship of the FLU-CIN Strategy Group (that includes JSN-V-T, WSL, MGS, PJMO, RCR, BLT, SJB, JMcM, JEE and KGN as members) was provided by the Department of Health, England. The FLU-CIN Strategy Group had final responsibility for the interpretation of findings and decision to submit for publication. The CAP cohort study was funded by an unrestricted educational grant from Wyeth (now Pfizer).

  • Competing interests JSN-V-T has received funding to attend influenza related meetings, lecture and consultancy fees and research funding from several influenza antiviral drug and vaccine manufacturers and is a former employee of SmithKline Beecham plc (now GlaxoSmithKline), Roche Products Ltd and Sanofi-Pasteur MSD. SJB has received consultancy fees from GlaxoSmithKline and Baxter. MGS and BB are advisors to the Department of Health, England. PJMO is a member of the European Scientific Working Group on Influenza (ESWI) which is funded by the pharmaceutical industry. JEE has received consultancy fees from GlaxoSmithKline and performed paid work for the Department of Health, England. KGN has received H5 avian influenza vaccines from Novartis and H1N1 pandemic influenza vaccines from GlaxoSmithKline and Baxter to facilitate MRC and NIHR-funded trials. He has received consultancy fees from Novartis and GlaxoSmithKline and lecture fees from Baxter. A colleague of KGN at the University Hospitals of Leicester NHS Trust was Principal Investigator and recipient of research funding from Roche on antiviral resistance and from Novartis on pandemic H1N1 vaccines. WSL has received an unrestricted educational grant from Wyeth (now Pfizer).

  • Ethics approval Before commencement, FLU-CIN procedures were reviewed by the Ethics and Confidentiality Committee of the National Information Governance Board for Health and Social Care in England and approved for collection, storage and use of personal data for surveillance purposes. Full ethical approval was obtained from the Nottingham Research Ethics Committee for the conduct of the CAP cohort study.

  • Provenance and peer review Not commissioned; externally peer reviewed.

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