You are here

Article Text

Article menu
Download PDFPDF
Cystic fibrosis
Inhibition of p38 mitogen activated protein kinase controls airway inflammation in cystic fibrosis
  1. V Raia1,
  2. L Maiuri2,
  3. C Ciacci3,
  4. I Ricciardelli2,
  5. L Vacca1,
  6. S Auricchio1,4,
  7. M Cimmino5,
  8. M Cavaliere5,
  9. M Nardone5,
  10. A Cesaro5,
  11. J Malcolm2,
  12. S Quaratino6,
  13. M Londei2
  1. 1Department of Pediatrics, University Federico II of Naples, Italy
  2. 2Institute of Child Health, University College London, London, UK
  3. 3Department of Gastroenterology, University Federico II of Naples, Italy
  4. 4European Laboratory for the Investigation of Food Induced Diseases, Naples, Italy
  5. 5Department of Otolaryngology, University Federico II of Naples, Italy
  6. 6Cancer Research UK, Clinical Centre, Southampton General Hospital, University of Southampton, UK
  1. Correspondence to:
    Professor M Londei
    Institute of Child Health, University College London, 30 Guilford Street, London WC1N 1EH, UK; m.londeiich.ucl.ac.uk

Abstract

Background: Cystic fibrosis (CF) airways are characterised by chronic inflammation, increased interleukin (IL)-8 secretion, and neutrophil activation which are considered the principal factors of morbidity and mortality in CF patients. Optimising management of this chronic inflammatory response is therefore a key issue of basic and clinical CF research. Several reports have addressed ways to manage CF airways inflammation, and an attractive therapeutic strategy may be the inhibition of the p38-mitogen activated protein kinase (p38-MAP-k) pathway.

Methods: A new ex vivo model was used to study the mucosal inflammatory response to environmental airways stimuli. Nasal biopsy tissues from CF patients and controls were cultured ex vivo for 20 minutes, 4 hours, and 24 hours in the presence of lipopolysaccharide (LPS) from Pseudomonas aeruginosa (PA) with and without the p38-MAP-k inhibitor SB203580. Quantitative mRNA assessment, immunohistochemistry, and Western blots were used to detect the expression and modulation of inflammatory markers.

Results: PA-LPS challenge induced a time dependent mucosal inflammation indicated by rapid epithelial activation, IL-8 release, COX-2 upregulation, and neutrophil migration to the upper mucosal layers. Some of these LPS induced changes (IL-8 release and neutrophil migration) were specific to CF tissues. SB203580 significantly controlled all LPS induced mucosal changes in CF tissues.

Conclusion: These findings provide a rationale and proof of principle for the potential use of p38-MAP-k inhibitors to control inflammation in patients with CF.

  • CF, cystic fibrosis
  • IL, interleukin
  • LPS, lipopolysaccharide
  • MAP-k, mitogen activated protein kinase
  • PA, Pseudomonas aeruginosa
  • interleukin 8
  • neutrophil
  • inflammation
  • cystic fibrosis
  • mitogen activated protein kinase

https://doi.org/10.1136/thx.2005.042564

Statistics from Altmetric.com

    Request Permissions

    If you wish to reuse any or all of this article please use the link below which will take you to the Copyright Clearance Center’s RightsLink service. You will be able to get a quick price and instant permission to reuse the content in many different ways.

    View Full Text

    Footnotes

    • Published Online First 30 June 2005

    • Funded by ARC-UK and ICH grants and the Italian Cystic Fibrosis Research Foundation, FFC Latina, grant #16/2004, CICRA UK, Cancer Research UK.

    • Competing interests: none declared.