Article Text

Download PDFPDF
Investigation of alveolar macrophage function using lucigenin-dependent chemiluminescence.
  1. A J Williams,
  2. P J Cole


    The method using lucigenin-dependent phagocytic chemiluminescence is described for the assessment of alveolar macrophage metabolic activity in response to stimulation by opsonised particles or soluble agents. The requirement for superoxide anion (O2-) in the production of chemiluminescence is suggested by inhibition (95%) using superoxide dismutase. The results obtained are correlated with those obtained using another method of detecting O2- release (r = 0.61; p less than 0.05) and are also related by regression analysis to polymorphonuclear leucocyte contamination of the alveolar macrophage suspension. This shows that alveolar macrophages produce lucigenin-dependent chemiluminescence of the same order of magnitude as do polymorphonuclear leucocytes.

    Statistics from

    Request Permissions

    If you wish to reuse any or all of this article please use the link below which will take you to the Copyright Clearance Center’s RightsLink service. You will be able to get a quick price and instant permission to reuse the content in many different ways.