Role of prostaglandin H synthase-2 in prostaglandin E2 formation in rat carrageenin-induced pleurisy

Prostaglandins. 1996 Jan;51(1):19-33. doi: 10.1016/0090-6980(95)00168-9.

Abstract

Rat carrageenin-induced pleurisy was used to clarify the role of prostaglandin H synthase (PGHS)-2 in acute inflammation. Intrapleural injection of 0.2 ml of 2% lambda-carrageenin induced accumulation of exudate and infiltration of leukocytes into the pleural cavity. When PGHS-1 and -2 proteins in the pleural exudate cells were analyzed by Western blot analysis, PGHS-2 was detectable from 1 hr after carrageenin injection. Its level rose sharply, remained high from 3 to 7 hr after injection, and then fell to near the detection limit. PGHS-1 was also detected, but kept almost the same level throughout the course of the pleurisy. Levels of prostaglandin (PG) E2 and thromboxane (TX) B2 in the exudate increased from hour 3 to hour 7, and then declined. Thus, the changes of the level of PGE2 were closely paralleled those of PGHS-2. The selective PGHS-2 inhibitors NS-398, nimesulide and SC-58125 suppressed the inflammatory reaction and caused a marked decrease in the level of PGE2 but not in those of TXB2 and 6-keto-PGF 1 alpha. These results suggest that the PGHS-2 expressed in the pleural exudate cells may be involved in PGE2 formation at the site of inflammation.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • 6-Ketoprostaglandin F1 alpha / metabolism
  • Animals
  • Anti-Inflammatory Agents / pharmacology
  • Azo Compounds / metabolism
  • Blotting, Western
  • Carrageenan / pharmacology
  • Cell Count / drug effects
  • Cells, Cultured
  • Coloring Agents / metabolism
  • Cyclooxygenase Inhibitors / pharmacology
  • Dexamethasone / pharmacology
  • Dinoprostone / metabolism
  • Excipients / pharmacology
  • Inflammation / metabolism
  • Isoenzymes / metabolism
  • Leukocytes / drug effects
  • Leukocytes / metabolism
  • Male
  • Nitrobenzenes / pharmacology
  • Pleura / metabolism
  • Prostaglandin-Endoperoxide Synthases / metabolism*
  • Pyrazoles / pharmacology
  • Rats
  • Rats, Sprague-Dawley
  • Sulfonamides / pharmacology
  • Thromboxane B2 / metabolism
  • Trypan Blue

Substances

  • Anti-Inflammatory Agents
  • Azo Compounds
  • Coloring Agents
  • Cyclooxygenase Inhibitors
  • Excipients
  • Isoenzymes
  • Nitrobenzenes
  • Pyrazoles
  • Sulfonamides
  • N-(2-cyclohexyloxy-4-nitrophenyl)methanesulfonamide
  • 1-((4-methylsulfonyl)phenyl)-3-trifluoromethyl-5-(4-fluorophenyl)pyrazole
  • pontamine sky blue
  • Thromboxane B2
  • 6-Ketoprostaglandin F1 alpha
  • Dexamethasone
  • Carrageenan
  • Prostaglandin-Endoperoxide Synthases
  • Trypan Blue
  • Dinoprostone
  • nimesulide