Abstract
HM-1:IMSS, a pathogenic strain of Entamoeba histolytica, and its mutant BG-3, identified by resistance to cytochalasin D, were tested for their capacity to: (i) secrete electron-dense granules; (ii) adhere and digest native type I collagen gels; and (iii) produce liver abscesses in new-born hamsters. The results demonstrate that the mutant has low adherence to collagen, low electron-dense granule secretion and collagenolytic activity, and low capacity to produce liver lesions in vivo, compared with the parental strain HM1:IMSS.
Publication types
-
Research Support, Non-U.S. Gov't
MeSH terms
-
Animals
-
Animals, Newborn
-
Cell Adhesion
-
Collagen
-
Collagenases / analysis*
-
Cricetinae
-
Cytochalasin D / pharmacology
-
Cytoplasmic Granules / metabolism*
-
Cytoskeleton / ultrastructure*
-
Drug Resistance / genetics
-
Entamoeba histolytica / drug effects
-
Entamoeba histolytica / enzymology
-
Entamoeba histolytica / genetics*
-
Entamoeba histolytica / pathogenicity
-
Entamoeba histolytica / ultrastructure
-
Liver Abscess, Amebic / parasitology
-
Mesocricetus
-
Mutation
-
Protozoan Proteins / analysis*
-
Virulence / genetics
Substances
-
Protozoan Proteins
-
Cytochalasin D
-
Collagen
-
Collagenases