Abstract
Primary monolayer cultures of cells from normal mouse kidney were initiated and maintained in serum-free medium supplemented with insulin, transferrin and prostaglandin E1. Renal epithelial cells grow to confluence without detectable growth of fibroblasts. These cells contain an active 25(OH)D3 1-hydroxylase with half-maximal formation of 1,25(OH)2D3 achieved at a substrate concentration of 13.3 nM. Activity of this enzyme is increased by low calcium medium or parathyroid hormone and decreased by high calcium medium, high phosphate medium or 1,25(OH)2D3.
Publication types
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Research Support, Non-U.S. Gov't
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Research Support, U.S. Gov't, P.H.S.
MeSH terms
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25-Hydroxyvitamin D3 1-alpha-Hydroxylase / metabolism*
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Alprostadil
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Animals
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Blood
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Cell Division / drug effects
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Cells, Cultured
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Culture Media
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Insulin / pharmacology
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Kidney / enzymology*
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Kidney / physiology
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Kinetics
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Male
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Mice
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Mice, Inbred C57BL
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Prostaglandins E / pharmacology
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Steroid Hydroxylases / metabolism*
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Transferrin / pharmacology
Substances
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Culture Media
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Insulin
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Prostaglandins E
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Transferrin
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Steroid Hydroxylases
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25-Hydroxyvitamin D3 1-alpha-Hydroxylase
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Alprostadil