Abstract
A new method for fiber typing based on staining for actomyosin Ca,Mg-ATPase is presented. Inclusion of ethanol in the medium enhanced the differentiation of type 2A and 2B fibers. With this technique, type 1, 2A, and 2B fibers can be distinguished in a single-step procedure for human biopsy samples.
Publication types
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Research Support, U.S. Gov't, Non-P.H.S.
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Research Support, U.S. Gov't, P.H.S.
MeSH terms
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Actomyosin / metabolism*
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Adenosine Triphosphatases / metabolism*
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Animals
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Frozen Sections
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Histocytochemistry
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Humans
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Hydrogen-Ion Concentration
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Methods
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Muscles / anatomy & histology
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Muscles / enzymology*
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Rabbits
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Rats
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Staining and Labeling
Substances
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Actomyosin
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Adenosine Triphosphatases