We previously have found that monocytes from patients with allergic rhinitis and/or asthma produce less PGE2 than cells from normal subjects in response to a histamine-induced lymphokine. In order to investigate this observation further, we measured the fatty acid content in the total phospholipids derived from the plasma, red cells, buffy coat cells, neutrophils, monocytes and lymphocytes of 27 allergic patients and 21 normal controls. There were no substantial differences between atopics and normals in the fatty acid analyses carried out for plasma and red cells. However, linoleic acid (18:2n-6) levels were elevated significantly in the buffy coat fraction, while arachidonic acid (20:4n-6) levels were reduced. Measurement of fatty acid levels after fractionation of the buffy coat population into neutrophils and monocytes yielded similar elevations in 18:2n-6 and reduced 20:4n-6. In contrast, lymphocytes appeared to have the reverse pattern, i.e., significantly reduced 18:2n-6 and elevated 20:4n-6 levels. These data suggest that atopic leukocytes may have altered essential fatty acid metabolism.