DNA probes bind non-specifically to eosinophils during in situ hybridization: carbol chromotrope blocks binding to eosinophils but does not inhibit hybridization to specific nucleotide sequences

S Patterson; J Gross; A D Webster

doi:10.1016/0166-0934(89)90124-9

DNA probes bind non-specifically to eosinophils during in situ hybridization: carbol chromotrope blocks binding to eosinophils but does not inhibit hybridization to specific nucleotide sequences

J Virol Methods. 1989 Feb;23(2):105-9. doi: 10.1016/0166-0934(89)90124-9.

Authors

S Patterson¹, J Gross, A D Webster

Affiliation

¹ Division of Immunological Medicine, Clinical Research Centre, Harrow, Middlesex, U.K.

PMID: 2723016
DOI: 10.1016/0166-0934(89)90124-9

Abstract

Eosinophils were found to bind radiolabelled DNA probes non-specifically during in situ hybridization. Pretreatment of cells with carbol chromotrope blocked non-specific binding without interfering with the recognition of specific nucleotide sequences.

MeSH terms

DNA Probes*
DNA, Viral / metabolism*
Eosinophils / metabolism*
Humans
Indicators and Reagents*
Nucleic Acid Hybridization*

Substances

DNA Probes
DNA, Viral
Indicators and Reagents