NLRP3 inflammasome activation by mitochondrial ROS in bronchial epithelial cells is required for allergic inflammation

S R Kim; D I Kim; S H Kim; H Lee; K S Lee; S H Cho; Y C Lee

doi:10.1038/cddis.2014.460

NLRP3 inflammasome activation by mitochondrial ROS in bronchial epithelial cells is required for allergic inflammation

Cell Death Dis. 2014 Oct 30;5(10):e1498. doi: 10.1038/cddis.2014.460.

Authors

S R Kim¹, D I Kim¹, S H Kim², H Lee¹, K S Lee¹, S H Cho³, Y C Lee¹

Affiliations

¹ Department of Internal Medicine, Research Center for Pulmonary Disorders, Chonbuk National University Medical School, Research Institute of Clinical Medicine of Chonbuk National University - Biomedical Research Institute of Chonbuk National University Hospital, Deokjin-gu, Jeonju, South Korea.
² Department of Product Strategy and Development, LG Life Sciences Ltd, Seoul, Korea.
³ Division of Allergy-Immunology, Department of Medicine, Northwestern University, Feinberg School of Medicine, Chicago, IL, USA.

Abstract

Abnormality in mitochondria has been suggested to be associated with development of allergic airway disorders. In this study, to evaluate the relationship between mitochondrial reactive oxygen species (ROS) and NLRP3 inflammasome activation in allergic asthma, we used a newly developed mitochondrial ROS inhibitor, NecroX-5. NecroX-5 reduced the increase of mitochondrial ROS generation in airway inflammatory cells, as well as bronchial epithelial cells, NLRP3 inflammasome activation, the nuclear translocation of nuclear factor-κB, increased expression of various inflammatory mediators and pathophysiological features of allergic asthma in mice. Finally, blockade of IL-1β substantially reduced airway inflammation and hyperresponsiveness in the asthmatic mice. These findings suggest that mitochondrial ROS have a critical role in the pathogenesis of allergic airway inflammation through the modulation of NLRP3 inflammasome activation, providing a novel role of airway epithelial cells expressing NLRP3 inflammasome as an immune responder.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Animals
Asthma / blood
Asthma / pathology
Bronchi / pathology*
Bronchoalveolar Lavage Fluid / cytology
Carrier Proteins / metabolism*
Caspase 1 / metabolism
Cells, Cultured
DNA, Mitochondrial / metabolism
Epithelial Cells / drug effects
Epithelial Cells / metabolism*
Heterocyclic Compounds, 4 or More Rings / pharmacology
Humans
Hypersensitivity / metabolism
Hypersensitivity / pathology*
Inflammasomes / metabolism*
Inflammation / metabolism
Inflammation / pathology
Interleukin-1beta / metabolism
Intracellular Space / metabolism
Lipopolysaccharides / pharmacology
Lung / pathology
Male
Mice
Middle Aged
Mitochondria / metabolism*
NLR Family, Pyrin Domain-Containing 3 Protein
Ovalbumin
Peroxidase / metabolism
Reactive Oxygen Species / metabolism*
Sulfones / pharmacology
Trachea / pathology

Substances

Carrier Proteins
DNA, Mitochondrial
Heterocyclic Compounds, 4 or More Rings
Inflammasomes
Interleukin-1beta
Lipopolysaccharides
NLR Family, Pyrin Domain-Containing 3 Protein
NLRP3 protein, human
NecroX-5
Nlrp3 protein, mouse
Reactive Oxygen Species
Sulfones
Ovalbumin
Peroxidase
Caspase 1