Sputum proteomics in inflammatory and suppurative respiratory diseases

Am J Respir Crit Care Med. 2008 Sep 1;178(5):444-52. doi: 10.1164/rccm.200703-409OC. Epub 2008 Jun 19.

Abstract

Rationale: Markers of inflammatory activity are important for assessment and management of many respiratory diseases. Markers that are currently unrecognized may be more valuable than those presently believed to be useful.

Objectives: To identify potential biomarkers of suppurative and inflammatory lung disease in induced sputum samples.

Methods: Induced sputum was collected from 20 healthy control subjects, 24 patients with asthma, 24 with chronic obstructive pulmonary disease, 28 with cystic fibrosis (CF), and 19 with bronchiectasis. Twelve patients with CF had sputum sampled before and after antibiotic therapy for an infective exacerbation. The fluid phase of induced sputum was analyzed by surface-enhanced laser desorption/ionization time-of-flight (SELDI-TOF) mass spectroscopy on three protein array surfaces. Some protein markers were selected for identification, and relevant ELISA assays sought. For 12 patients with CF, both SELDI-TOF and ELISA monitored changes in inflammatory responses during infective exacerbations.

Measurements and main results: SELDI-TOF identified potential biomarkers that differentiated each of the disease groups from healthy control subjects: at a significance of P < 0.01, there were 105 for asthma, 113 for chronic obstructive pulmonary disease, 381 for CF, and 377 for bronchiectasis. Peaks selected for protein identification yielded calgranulin A, calgranulin B, calgranulin C, Clara cell secretory protein, lysosyme c, proline rich salivary peptide, cystatin s, and hemoglobin alpha. On treatment of an infective CF exacerbation, SELDI-TOF determined falls in levels of calgranulin A and calgranulin B that were mirrored by ELISA-measured falls in calprotectin (heterodimer of calgranulins A and B).

Conclusions: Proteomic screening of sputum yields potential biomarkers of inflammation. The early development of a clinically relevant assay from such data is demonstrated.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Adult
  • Aged
  • Asthma / diagnosis
  • Biomarkers / metabolism*
  • Bronchial Diseases / diagnosis*
  • Bronchiectasis / diagnosis
  • Case-Control Studies
  • Cystic Fibrosis / diagnosis
  • Female
  • Humans
  • Inflammation
  • Leukocyte L1 Antigen Complex / metabolism
  • Longitudinal Studies
  • Lung Diseases / diagnosis*
  • Male
  • Middle Aged
  • Peptide Mapping* / methods
  • Proteomics
  • Pulmonary Disease, Chronic Obstructive / diagnosis
  • Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization
  • Sputum / chemistry*
  • Suppuration

Substances

  • Biomarkers
  • Leukocyte L1 Antigen Complex