Chest
Clinical InvestigationsEPIDEMIOLOGY AND DIAGNOSISHuman Leukocyte Antigen-Associated Susceptibility to Pulmonary Tuberculosis: Molecular Analysis of Class II Alleles by DNA Amplification and Oligonucleotide Hybridization in Mexican Patients
Section snippets
Study Population
Fifty randomly selected nonimmunosuppressed patients with pulmonary TB (PTB) were included in this study. The study subjects were unrelated Mexican patients who were residents of Mexico City and were hospitalized at the National Institute of Respiratory Diseases at the time of the study. In all cases, the diagnosis of TB was confirmed by light microscopy, which revealed the presence of acid-fast bacilli in sputum, and by culture of the M tuberculosis.
The criteria for considering patients with
Results
The study included 50 nonimmunosupressed patients (with a mean[± SD] age of 39.1 ± 13.0 years). Evaluated as control subjects were 95 healthy individuals (35.4 ± 10.1 years of age), 37 HIV-infected patients without infectious or neoplastic disease (31 ± 8.9 years of age), 15 immunosuppressed (HIV-infected) patients with TB (31.1 ± 5.7 years of age), and 20 healthy non-HIV-infected homosexual subjects (26.3 ± 8.5 years of age). The latter control group displayed no significant differences in HLA
Discussion
Molecular typing has proven useful for clarifying the genetic susceptibility associated with the HLA system. Tissue or cell sampling may now be achieved by examining the cell nucleic acid rather than by using serologic methods, thus increasing the sensitivity of the sampling. In our study, PCR, in combination with the sequence-specific oligonucleotide probes, clearly showed an association between the frequencies of the DQA1*0101, DQB1*0501, and DRB1*1501 alleles and PTB. The aforementioned
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