Mechanisms of asthma and allergic inflammationFunctional haplotypes of IL-12B are associated with childhood atopic asthma
Section snippets
Study subjects
All subjects with asthma were diagnosed according to the criteria of the National Institutes of Health22 and demonstrated at least 12% improvement in their FEV1 measurement after β2-agonist inhalation. The diagnosis of atopic asthma was based on 1 or more positive skin scratch test responses to seven common aeroallergens in the presence of a positive histamine control and a negative vehicle control. The seven aeroallergens were house dust, Felis domestics dander (Fel d), Canis familiaris
Results
We identified 13 biallelic polymorphisms in IL-12B: 4 in the 5′ flanking region, 1 in the 3′ UTR, and 8 in the intron (Table I and Fig 1). Seven polymorphisms were contained in the public databases available at Web sites. 10841C>A in exon 8 was reported previously as 1188C>A.24 Rare SNPs with minor allele frequencies of 2% were not included in the analysis. Pairwise LD among 8 SNPs with a frequency >0.15 was measured by different parameters, |D′| and r2 (Table II). D′ is inversely biased with
Discussion
We showed here a significant association between asthma susceptibility and an SNP in the 3′ UTR region, 10841C>A, and promoter polymorphism −6415 CTCTAA>GC was also associated with asthma. Furthermore, we found a haplotype that affected the stability of transcripts and enhanced the transcriptional level of IL-12B. Although the functional effects of these 2 polymorphisms were analyzed in this study, the polymorphisms that are in linkage disequilibrium with these 2 variants were not examined.
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Association of interleukin-12B gene polymorphisms and mRNA expression with preeclampsia
2022, European Journal of Obstetrics and Gynecology and Reproductive BiologyInterleukin-12B+1188A/C polymorphism contributes to increased hepatocellular carcinoma susceptibility: Evidence from a meta-analysis
2014, Clinics and Research in Hepatology and GastroenterologyCitation Excerpt :The wild type ±1188AA genotype was associated with relatively higher expression of IL-12 cytokine than the AC and the CC genotypes in EBV-transformed human cell lines [16]. Moreover, IL-12B mRNA from the ±1188A allele was more stable than that from the ±1188 C allele [17]. Based on the important biological function of IL-12B +1188A/C polymorphism, several molecular epidemiological studies have been performed to investigate the effect of IL-12B +1188A/C polymorphism on HCC risk, but the results were inconsistent [7,18–21].
Implications of cytokine genes in allergic asthma
2014, Allergologia et ImmunopathologiaCitation Excerpt :Moreover, the IL12B gene is also a good candidate in view of the genomic region in which it is located, i.e. alongside other asthma-linked genes. The polymorphism in position 1888 3′UTR (rs3212227) has been associated to childhood asthma.11 Interferon gamma (IFN-γ) or type II interferon is a cytokine that plays a critical role in the innate and adaptive immune response to viral or intracellular bacterial infections, as well as in tumour control.
Genetic variations in interleukin-12 related genes in immune-mediated diseases
2012, Journal of Autoimmunity
Supported by grants-in-aid from the Ministry of Health, Labor and Welfare; Japan Science and Technology Corp; and the Japanese Millennium project.