Basic and clinical immunologyAntigen presentation of the immunodominant T-cell epitope of the major mugwort pollen allergen, Art v 1, is associated with the expression of HLA-DRB1∗01
Section snippets
Characterization of patients allergic to mugwort
Fifty-one patients with mugwort pollen allergy (27 female and 24 male; mean age, 34.5 years; age range, 12-65 years) were included in this study. The diagnosis of mugwort allergy was based on typical clinical history, ie, recurrent rhinitis/conjunctivitis during late summer and positive skin prick tests (wheal diameter >5 mm) to mugwort pollen extract (ALK, Horsholm, Denmark). The CAP-RAST test for mugwort pollen (w6; Pharmacia Diagnostics, Uppsala, Sweden) was positive for all patients (mean
Immunodominance of Art v 125-36 in T-cell responses
All 51 patients included in this study had clinically defined mugwort pollen allergy and possessed serum IgE specific for nArt v 1 and rArt v 1, as determined by immunoblot and ELISA (not shown). In addition, nArt v 1 and rArt v 1 induced proliferation in PBMCs, oligoclonal TCLs, or TCCs derived from all patients. Similar proliferative responses were obtained in TCLs stimulated with nArt v 1, rArt v 1, and Art v 125-36 (mean ± SD: 41,066 ± 27,849 cpm, 44,104 ± 30,626 cpm, and 46,136 ± 30,626
Discussion
Mugwort pollen allergy is frequently encountered in Central Europe, and sensitization to the major allergen Art v 1 is observed in the vast majority of patients. Individuals allergic to mugwort who display IgE against Art v 1 also respond to the immunodominant peptide Art v 125-36 with their T cells and a typical TH2 response.11 In this study, the minimal epitope and essential amino acids within Art v 125-36 were characterized, and their restriction by HLA-DR was demonstrated. The T-cell
References (34)
- et al.
Celery allergens in patients with positive double-blind placebo-controlled food challenge
J Allergy Clin Immunol
(2000) - et al.
Identification of common allergenic structures in mugwort and ragweed pollen
J Allergy Clin Immunol
(1998) - et al.
Native Art v 1 and recombinant Art v 1 are able to induce humoral and T cell-mediated in vitro and in vivo responses in mugwort allergy
J Allergy Clin Immunol
(2003) The role of lymphocytes in allergic disease
J Allergy Clin Immunol
(2000)- et al.
T-cell responses to allergens: epitope-specificity and clinical relevance
Immunol Today
(1996) - et al.
Allergen immunotherapy: therapeutic vaccines for allergic diseases. A WHO position paper
J Allergy Clin Immunol
(1998) - et al.
Immunotherapy with Fel d 1 peptides decreases IL-4 release by peripheral blood T cells of patients allergic to cats
J Allergy Clin Immunol
(1998) - et al.
The safety and efficacy of ALLERVAX CAT in cat allergic patients
Clin Immunol
(1999) - et al.
Successful immunotherapy with T-cell epitope peptides of bee venom phospholipase A2 induces specific T-cell anergy in patients allergic to bee venom
J Allergy Clin Immunol
(1998) - et al.
Allergen-specific T-cell tolerance induction with allergen-derived long synthetic peptides: results of a phase I trial
J Allergy Clin Immunol
(2003)
City spore concentrations in the European Economic Community (EEC). IV. Summer weed pollen (Rumex, Plantago, Chenopodiaceae, Artemisia), 1976 and 1977
Clin Allergy
Artemisia
Celery allergy associated with birch and mugwort pollinosis
Allergy
IgE cross-reactivity between birch pollen, mugwort pollen and celery is due to at least three distinct cross-reacting allergens: immunoblot investigation of the birch-mugwort-celery syndrome
Clin Exp Allergy
Molecular characterization of Api g 1, the major allergen of celery (Apium graveolens), and its immunological and structural relationships to a group of 17-kDa tree pollen allergens
Eur J Biochem
Molecular and immunological characterization of profilin from mugwort pollen
Biol Chem
Art v 1, the major allergen of mugwort pollen, is a modular glycoprotein with a defensin-like and a hydroxyproline-rich domain
FASEB J
Cited by (52)
Maternal allergen-specific IgG might protect the child against allergic sensitization
2019, Journal of Allergy and Clinical ImmunologyMolecular Aspects of Allergens and Allergy
2018, Advances in ImmunologyAllergens of weed pollen: An overview on recombinant and natural molecules
2014, MethodsCitation Excerpt :At the T cell level, no differences in proliferation were observed in PBMC or allergen-specific T cell lines or clones stimulated with recombinant or natural Art v 1 [34]. Notably, T cell responses to Art v 1 are driven by a single immunodominant and HLA-DRB∗01-restricted T cell epitope (Art v 125–36) shown to be recognized by 96% of mugwort allergic patients [35]. A Jurkat T cell line expressing the human T cell receptor specific for Art v 125–36 has been constructed and will enable the establishment of in vivo systems for studying allergen-specific T cell interactions influencing the allergic sensitization [36].
Bet v 1-specific T-cell receptor/forkhead box protein 3 transgenic T cells suppress Bet v 1-specific T-cell effector function in an activation-dependent manner
2011, Journal of Allergy and Clinical Immunology
Supported by the Fonds zur Förderung der wissenschaftlichen Forschung, Austria (FSP S8802-MED, S8808-MED, and SFB F1807), and Biomay, Austria.