Reassessment of FDG uptake in tumor cells: High FDG uptake as a reflection of oxygen-independent glycolysis dominant energy production

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Abstract

To determine appropriate use of 2-[18F]-fluoro-2-deoxy-d-glucose (FDG) in the diagnosis of malignant tumors, the mechanism of enhanced FDG uptake in tumor cells was reassessed using in vitro cultured cell lines and 3H-deoxyglucose (DG), in combination with possible parameters of aerobic and anaerobic energy production. The high DG uptake in the tumor cells reflected the dependency of energy production on anaerobic glycolysis, and paradoxically on low levels of aerobic oxidative phosphorylation in mitochondria. We discuss here factors underlying anaerobic glycolysis in tumor cells.

References (32)

  • A.C. Clavo

    Fluorodeoxyglucose uptake in human cancer cell lines is increased by hypoxia

    J. Nucl. Med.

    (1995)
  • G. DiChiro

    Glucose utilization by intracranial meningiomas as an index of tumor aggressively and probability of recurrence: A PET study

    Radiology

    (1987)
  • E. Eigenbrodt et al.

    New perspectives on carbohydrate metabolism in tumor cells

  • T.G. Graeber

    Hypoxia-mediated selection of cells with diminished apoptotic potential in solid tumors

    Nature

    (1996)
  • K. Higashi

    Does FDG uptake measure proliferative activity of human cancer cells? in vitro comparison wtih DNA flow cytometry and tritiated thymidine uptake

    J. Nucl. Med.

    (1993)
  • K. Higashi

    In vitro assessment of 2-fluoro-2-deoxy-d-glucose, L-methionine and Thymidine as agents to monitor the early response of a human adenocarcinoma cell line to radiotheraoy

    J. Nucl. Med.

    (1993)
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