A new murine model of pulmonary eosinophilic hypersensitivity: Contribution to experimental asthma☆,☆☆,★,★★
Section snippets
Animals
F1(BALB/c x A/J), BALB/c, A/J, C57Bl/6J, and CH3/HePas mice (6 to 8 weeks of age) were obtained from our own animal facilities.
Heat-coagulated hen's egg white
Separated hen's egg white was placed in a water bath at 100° C for 30 minutes. The solidified egg white was washed in distilled water, dehydrated in 100% ethanol for 48 hours, and cut into small fragments of 4 × 2 × 2 mm, weighing about 40 mg each. The egg white fragments were rehydrated in Dulbecco's phosphate-buffered saline (PBS) for 2 hours before implantation.
Heat-aggregated ovalbumin
Cells in the BAL
Groups of F1 (BALB/c X A/J) mice were immunized with OA-AL or with EWI and 14 days later challenged intratracheally with aggregated ovalbumin. Fig. 1 shows that 48 hours after antigen challenge, the total number of cells in the BAL increased significantly in OA-AL and EWI-immunized groups in comparison with nonimmunized mice.
Discussion
Asthma is a chronic lung disease that is increasing in industrialized countries. A prominent eosinophil infiltrate in the airway tissue is one of the characteristic features of asthma.25 Thus it is of interest to develop experimental models that mimic this disease. Although several animal models have been described in the literature,21 all of them require adjuvants in the immunization protocols, and some of them do not present a typical allergic LPR as it occurs in human beings. We have
Acknowledgements
We thank Dr. Paulo Hilario Saldiva from the Department of Pathology, School of Medicine, University of São Paulo for the careful histopathologic analysis of the lungs and many helpful discussions.
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From the Department of Immunology, Institute of Biomedical Sciences, University of São Paulo. Sandra Facincone and Ana Lúcia Pereira de Siqueira are postgraduate students from the Department of Pathology, School of Veterinary Medicine and the Department of Immunology, respectively, of the University of São Paulo.
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Supported by Fundaças de Ampara a Pesquisa do Estado de São Paulo (FAPESP) and Conselho Nacional de Pesquisa (CNPq).
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Reprint requests: Sonia Jancar, BSc, PhD, Departamento de Imunologia, Instituto de Ciências Biomédicas, Universidade de São Paulo, Av. Prof. Lineu Prestes 2415, 05508-900, São Paulo, Brazil.
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