Elsevier

Biochemical Pharmacology

Volume 27, Issue 5, 1 March 1978, Pages 773-775
Biochemical Pharmacology

Research paper
Direct extraction radioassay for cathechol-o-methyl-transferase activity

https://doi.org/10.1016/0006-2952(78)90519-1Get rights and content

Abstract

An improved radiochemical assay for catechol-O-methyl-transferase is presented that is particularly suitable for use with large numbers of samples. 3,4-Dihydroxyphenylacetic acid is converted to radioactively-labelled homovanillic acid in the presence of s-adenosyl-l-[methyl-3H]methionine. This product is extracted into an organic solvent-scintillant mixture and scintillation counting is performed without further manipulation.

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Present address: Department of Biochemistry, Trinity College, Dublin, 2, Eire.

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