Inhibition of prostaglandin synthesis in man

doi:10.1016/0006-291X(72)90470-6

Biochemical and Biophysical Research Communications

Volume 49, Issue 3, 1 November 1972, Pages 720-726

https://doi.org/10.1016/0006-291X(72)90470-6 Get rights and content

Abstract

7α-Hydroxy-5,11-diketotetranor-prostane-1,16-dioic acid, the major urinary metabolite of prostaglandins E₁ and E₂ in man, was determined in human urine by a method based on the use of the bis (O-²H₃-methyloxime) derivative of dimethyl 7α-hydroxy-5,11-diketotetranor-prostane-1,16-dioate as internal standard and determination of the ratio between unlabeled and deuterium-labeled molecules by multiple-ion analysis. Male subjects excreted larger amounts of the metabolite (6.5–46.7 μg/24 hours, n=10) than did female subjects (2.5–5.3 μg/24 hours, n=10). The excretion rate was strongly suppressed following oral administration of therapeutic doses of indomethacin, aspirin and sodium salicylate.

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Inhibition of prostaglandin synthesis in man

Abstract

J. Biol. Chem

Lancet

Anal. Biochem

Biochem. Biophys. Res. Commun

J. Biol. Chem

J. Amer. Chem. Soc