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In situ Microrheological Determination of Neutrophil Stiffening Following Adhesion in a Model Capillary

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Abstract

There has been considerable debate on the relative importance of biochemical stimuli and mechanical deformation in neutrophil adhesion in lung capillaries, a process observed following bacterial infection in the body. In contrast to venules, where the vessel diameter is larger than the leukocyte diameter (6–9 μm) and the adhesion process is better understood, in lung capillaries the vessel diameter (2–8 μm) is smaller than the leukocyte diameter. In this study, a micropipette was used as a model for the alveolar capillary microcirculation, allowing the effects of adhesion molecules (ICAM-1) on cell mechanical properties to be observed while applying a mechanical deformation. The microrheology technique that tracks the thermal motion of granules within neutrophils was used to extract the local intracellular viscoelastic moduli. Small regional differences in rheology were found, with the central body region being significantly stiffer than the leading end cap region. When cells were exposed to ICAM-1, the regional differences were preserved, but the viscoelastic moduli were moderately increased in all regions. These results are consistent with the literature on leukocyte sequestration and provide insight into the regional rheological effects of deformation and adhesion molecules on neutrophils.

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Abbreviations

BSA:

Bovine Serum Albumin

DPBS:

Dulbecco’s Phosphate Buffered Saline

HBSS:

Hanks’ Balanced Salt Solution

ICAM-1:

Inter Cellular Adhesion Molecule-1

ID:

Inner Diameter

MSD:

Mean Squared Displacement

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Acknowledgments

This work was funded by an award from the American Heart Association 0465268B and by a National Science Foundation CAREER award (BES-0547165).

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Authors and Affiliations

  1. Department of Chemical and Biomolecular Engineering, Ohio University, Athens, OH, 45701, USA

    Anand Pai, Prithu Sundd & David F. J. Tees

  2. Department of Physics & Astronomy, Ohio University, 251A Clippinger Labs, Athens, OH, 45701, USA

    David F. J. Tees

Authors
  1. Anand Pai
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  2. Prithu Sundd
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  3. David F. J. Tees
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Correspondence to David F. J. Tees.

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Pai, A., Sundd, P. & Tees, D.F.J. In situ Microrheological Determination of Neutrophil Stiffening Following Adhesion in a Model Capillary. Ann Biomed Eng 36, 596–603 (2008). https://doi.org/10.1007/s10439-008-9437-8

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  • DOI: https://doi.org/10.1007/s10439-008-9437-8

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