Abstract
The purpose of this study was to assess the effect of passive cigarette smoking on the oxidative and anti-oxidative status of plasma in infants. Eighty-four infants aged 6–28 weeks were divided into two groups: the study group included infants who had been exposed to passive smoking via at least five cigarettes per day for at least the past 6 weeks at home, while the control group included infants who had never been exposed to passive smoking. The antioxidative status of plasma was assessed by the measurement of individual antioxidant components: vitamin C, albumin, bilirubin, uric acid, thiol contents and total antioxidant capacity (TAC 1 and TAC 2). Oxidative status was assessed by the determination of total peroxide levels and the oxidative stress index (OSI 1 and OSI 2). Plasma vitamin C, thiol concentration and TAC 1 and TAC 2 levels were significantly lower, whereas plasma total peroxide levels and OSI 1 and OSI 2 were significantly higher, in passive smoking infants than in the controls (P<0.01). We conclude that passive smoking has a negative impact on numerous parts of the antioxidant defence system in infants, and exposes them to potent oxidative stress.
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Ahn MR, Kumazawa S, Hamasaka T, Bang KS, Nakayama T (2004) Antioxidant activity and constituents of propolis collected in various areas of Korea. J Agric Food Chem 52:7286–7292
Alberg AJ (2002) The influence of cigarette smoking on circulating concentrations of antioxidant micronutrients. Toxicology 180:12
Benzie IF, Strain JJ (1999) Ferric reducing/antioxidant power assay: direct measure of total antioxidant activity of biological fluids and modified version for simultaneous measurement of total antioxidant power and ascorbic acid concentration. Methods Enzymol 299:15–27
Brown AJ (1998) Paradoxical effects of acute cigarette smoking on plasma antioxidant status in humans. Nutr Res 18:1499–1519
Cao G, Prior RL (1998) Comparison of different analytical methods for assessing total antioxidant capacity of human serum. Clin Chem 44:1309–1315
Cross CE, O’Neill CA, Reznick AZ, Hu ML, Marcocci L, Packer L, Frei B (1993) Cigarette smoke oxidation of human plasma constituents. Ann N Y Acad Sci 686:72
Dietrich M, Block G, Norkus EP, Hudes M, Traber MG, Cross CE, Packer L (2003) Smoking and exposure to environmental tobacco smoke decrease some plasma antioxidants and increase tocopherol in vivo after adjustment for dietary antioxidant intakes. Am J Clin Nutr 77:160–166
Durak I, Elgun S, Kemal Bingol N, Burak Cimen MY, Kacmaz M, Buyukkocak S, Serdar Ozturk H (2002) Effects of cigarette smoking with different tar content on erythrocyte oxidant/antioxidant status. Addict Biol 7:255
Erel O. (2004) A novel automated direct measurement method for total antioxidant capacity using a new generation, more stable ABTS radical cation. Clin Biochem 37:277–285
Erel O (2004) A novel automated method to measure total antioxidant response against potent free radical reactions. Clin Biochem 37:112–119
Frei B, Forte TM, Ames BN, Cross CE (1991) Gas phase oxidants of cigarette smoke induce lipid peroxidation and changes in lipoprotein properties in human blood plasma. Biochem J 277:133–138
Halliwell B (1996) Antioxidants in human health and disease. Annu Rev Nutr 16:33–50
Harats D, Ben-Naim M, Dabach Y, Hollander G, Stein O, Stein Y (1989) Cigarette smoking renders LDL susceptible to peroxidative modifications and enhanced metabolism by macrophages. Atherosclerosis 79:245–252
Harma M, Harma M, Erel O (2003) Increased oxidative stress in patients with hydatidiform mole. Swiss Med Wkly 133:563–566
Harma M, Harma M, Erel O (2005) Oxidative stress in women with preeclampsia. Am J Obstet Gynecol 192:656–657
Harma M, Harma M, Erel O (2005) Measurement of the total antioxidant response in preeclampsia with a novel automated method. Eur J Obstet Gynecol Reprod Biol 118:47–51
Joshua E, Muscat JE, Kleinma W, Colosimo S, Muir A, Lazarus P (2004) Enhanced protein glutathione and oxidative stress in cigarette smoking. Free Radic Biol Med 36:464–470
Kelly G (2003) The Interaction of cigarette smoking and antioxidants. Part III: ascorbic acid. Altern Med Rev 8:43–54
Kim SH, Kim JS, Shin HS, Keen CL (2003) Influence of smoking on markers of oxidative stress and serum mineral concentrations in teenage girls in Korea. Nutrition 19:240–243
Kosecik M, Erel O, Sevinc E, Selek S (2005) Increased oxidative stress in children exposed to passive smoking. Int J Cardiol 100:61–64
Liu X, Lu J, Liu S (1999) Synergistic induction of hydroxyl radical-induced DNA single-strand breaks by chromium (VI) compound and cigarette smoke solution. Mutat Res 440:109
McCall MR, van den Berg JJM, Kuypers FA, Tribble DL, Krauss RM, Knoff LJ, Forte TM (1994) Modification of LCAT activity and HDL structure. New links between cigarette smoke and coronary heart disease. Arterioscler Thromb 14:248–253
Michael PR (2000) Cigarette smoking, endothelial injury and cardiovascular disease. Int J Exp Pathol 81:219
Miyazawa T (1989) Determination of phospholipid hydroperoxides in human blood plasma by a chemiluminescence-HPLC assay. Free Radic Biol Med 7:209–217
Polidori MC, Mecocci P, Stahl W, Sies H (2003) Cigarette smoking cessation increases plasma levels of several antioxidant micronutrients and improves resistance towards oxidative challenge. Br J Nutr 90:147–150
Prior RL, Cao G (1999) In vivo total antioxidant capacity: comparison of different analytical methods. Free Radic Biol Med 27:1173–1181
Rahman I, MacNee W (1996) Oxidant/antioxidant imbalance in smokers and chronic obstructive pulmonary disease. Thorax 51:348
Reibel J (2003) Tobacco and oral diseases. Update on the evidence, with recommendations. Med Princ Pract 12:22
Scheffler E, Wiest E, Woehrle J, Otto I, Schulz I, Huber L, Ziegler R, Dressel HA (1992) Smoking influences the atherogenic potential of low-density lipoprotein. Clin Investig 70:263–268
Schwertner HA (1998) Association of smoking and low serum bilirubin antioxidant concentrations. Atherosclerosis 136:383–387
Strauss RS (2001) Environmental tobacco smoke and serum vitamin C levels in children. Pediatrics 107:540–542
Therriault MJ, Proulx LI, Castonguay A, Bissonnette EY (2003) Immunomodulatory effects of the tobacco-specific carcinogen, NNK, on alveolar macrophages. Clin Exp Immunol 132:232
Yanik M, Erel O, Kati M (2004) The relationship between potency of oxidative stress and severity of depression. Acta Neuropsychiatr 16:200–203
Yeni E, Gulum M, Selek S, Erel O, Unal D, Verit A Savas M (2005) Comparison of oxidative/antioxidative status of penile corpus cavernosum blood and peripheral venous blood. Int J Impot Res 17:19–22
Yildiz L, Kayaoglu N, Aksoy H (2002) The changes of superoxide dismutase, catalase and glutathione peroxidase activities in erythrocytes of active and passive smokers. Clin Chem Lab Med 40:612
Yokode M, Kita T, Arai H, Kawai C, Narumiya S, Fujiwara M (1988) Cholesteryl ester accumulation in macrophages incubated with low density lipoprotein pretreated with cigarette smoke extract. Proc Natl Acad Sci U S A 85:2344–2348
Yoshie Y, Ohshima H (1997) Synergistic induction of DNA strand breakage by cigarette tar and nitric oxide. Carcinogenesis 18:1359
Acknowledgements
We are most grateful to the technical staff of the Laboratory Unit, Children’s Hospital and Harran University Medical School for their assistance in conducting this study.
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Aycicek, A., Erel, O. & Kocyigit, A. Increased oxidative stress in infants exposed to passive smoking. Eur J Pediatr 164, 775–778 (2005). https://doi.org/10.1007/s00431-005-1720-1
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DOI: https://doi.org/10.1007/s00431-005-1720-1