Abstract
Lyt-2+ T cells constitute a significantly greater proportion of the total peripheral T-cell population in C57BL mice than in BALB/c and other mouse strains. The inheritance of this differential representation of Lyt-2− vs. Lyt-2+ T cells was studied by two-color immunofluorescence analysis of peripheral T cell subsets in BALB/c, C57BL, F1 and F2 generations, and in CXB recombinant inbred strains. It was shown that the C57BL phenotype (low Lyt-2−/Lyt-2+ ratio) is a dominant Mendelian character. Studies of subpopulations of thymocytes and of early thymus emigrants indicate that the representation of mature Lyt-2− and Lyt-2+ T cells is influenced by mechanisms of selection or differential turnover in the peripheral lymphoid organs, but that thymic and prethymic influences may also play a role.
Similar content being viewed by others
References
Bailey, D. W.: Recombinant inbred strains. An aid to finding identify, linkage, and function of histocompatibility and other genes. Transplantation 11: 325–327, 1971
Barth, W. F., McLaughlin, C. L., and Fahey, J. L.: The immunoglobulins of mice. VI. Response to immunization. J. Immunol. 95: 781–790, 1965
Braley, H. C. and Freeman, M. J.: Strain differences in the antibody plaque-forming cell responses of inbred mice to pneumococcal polysaccharide. Cell Immunol. 2: 73–81, 1971
Bryant, B. J.: Renewal and fate in mammalian thymus — mechanisms and inferences of thymocytokinetics. Eur. J. Immunol. 2: 30–45, 1972
Butcher, E. C., Rouse, R. V., Coffman, R. L., Nottenburg, C. N., Hardy, R. R., and Weissman, I. L.: Surface phenotype of Peyer's patch germinal center cells: implications for the role of germinal center cells in B cell differentiation. J. Immunol. 129: 1698–2707, 1982
Cantor, H. and Boyse, E. A.: Functional subclasses of T lymphocytes bearing Ly antigens. II. Cooperation between subclasses of Lyt cells in the generation of killer activity. J. Exp. Med. 141: 1390–1399, 1975
Cantor, H. and Boyse, E. A.: Regulation of the immune response by T cell subclasses In O. Stutman (ed.): Contemporary Topics in Immunobiology, Vol. 7, pp. 47–67, New York Plenum Press, 1977
Click, R. E., Benck, L., Alter, B. J. and Lovchick, J. C.: Immune responses in vitro. VI. Genetic control of the in vivo-in vitro discrepancies in IgS antibody synthesis. J. Exp. Med. 136: 1241–1257, 1972
Hollander, N., Pillemer, E., and Weissman, I. L.: Blocking effect of Lyt-2 antibodies on T cell functions. J. Exp. Med. 152: 674–687, 1980
Imanishi, T. and Mäkela, O.: Inheritance of antibody specificity. II. Anti- (4-hydroxy-s-bromo-3nitrophenyl) acetyl in the mouse. J. Exp. Med. 141: 240–254, 1975
Jandinski, J., Cantor, H., Tadakuma, T., Peavy, D. L., and Pierce, C. W.: Separation of helper cells from suppressor T cells expressing different Ly components. Polyclonal activation: suppressor and helper activities are inherent properties of distinct T cell subclasses. J. Exp. Med. 143: 1382–1390, 1976
Kraal, G., Weissman, I. L., and Butcher, E. C.: Differences in migration and in situ distribution of T cell subsets in mucosal and non-mucosal lymphoid organs. J. Immunol. 130: 1097–1102, 1983
Minga, U. M., Segre, M., and Segre, D.: Numbers and avidity of anti-DNP antibody plaques in different inbred mouse strains. Immunogenetics 2: 369–377, 1975
Raulet, D. H., Gottlieb, P. D., and Bevan, M. J.: Fractionation of lymphocyte populations with monoclonal antibodies specific for Lyt-2:2 and Lyt-3.1. J. Immunol. 125: 1136–1143, 1980
Schroff, R. W., Gottlieb, M. S., Prince, H. E., Chai, L. L., and Fahey, J. L.: Immunological studies of homosexual men with immunodeficiency and Kaposi's sarcoma. Clin. Immun. Immunopath. 27: 300–314, 1983
Scollay, R. and Weissman, I. L.: T cell maturation: thymocyte and thymus migrant subpopulations defined with monoclonal antibodies to the antigens Lyt-1, Lyt-2 and ThB. J. Immunol. 124: 2841–2844, 1980
Scollay, R., Kochen, M., Butcher, E. C., and Weissman, I. L.: Lyt markers on thymus cell migrants. Nature 276: 79–80, 1978
Scollay, R., Jacobs, S., Jerabek, L., Butcher, E. C., and Weissman, I. L.: T cell maturation: thymocyte and thymus migrant subpopulations defined with monoclonal antibodies to MHC region antigens. J. Immunol. 124: 2845–2853, 1980
Seman, M., Zilberfarb, V., Stanislawski, M., and Dubert, J. M.: Genetic control of IgG2a production in the response to sheep erythrocytes. Immunogenetics 7: 131–139, 1978
Seman, M. and Zilberfarb, V.: Genetic control of the IgG2a response to sheep erythrocytes in mice: isotype- and antigen-specific T cell-mediated suppression in low responders. J. Immunol. 122: 2534–3540, 1979
Seman, M. and Zilberfarb, V.: Complementation between a gene of the I-E subregion and a non-H-2 gene in the class-specific suppression of IgG2a antibody to sheep erythrocytes. Immunogenetics 12: 89–99, 1981
Silver, D. M. and Winn, H. J.: Variations in the responses of C57BL and A mice to sheep red blood cells. Cell Immunol. 7: 237–245, 1973
Swain, S. L.: Significance of Lyt phenotypes. Lyt-2 antibodies block activities of T cells that recognize class I major histocompatibility complex antigens regardless of their function. Proc. Natl. Acad. Sci. U.S.A. 78: 7101–7105, 1981
Swain, S. L. and Panfili, P. R.: Helper cells activated by allogeneic H-2K or H-2D differences have a Ly phenotype distinct from those responsive to I differences. J. Immunol. 122: 383–391, 1979
Takahashi, T., Old, L. J., and Boyse, E. A.: Surface alloantigens of plasma cells. J. Exp. Med. 131: 1323–1341, 1970
Weissman, I. L.: Thymus cell maturation: studies on the origin of cortisone-resistant thymic lymphocytes. J. Exp. Med. 137: 504–510, 1973
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Kraal, G., Weissman, I.L. & Butcher, E.C. Genetic control of T-Cell subset representation in inbred mice. Immunogenetics 18, 585–592 (1983). https://doi.org/10.1007/BF00345966
Received:
Issue Date:
DOI: https://doi.org/10.1007/BF00345966