Detection and Quantification of the Leptin Receptor Splice Variants Ob-Ra, b, and, e in Different Mouse Tissues

doi:10.1006/bbrc.1997.7205

Biochemical and Biophysical Research Communications

Volume 238, Issue 2, 18 September 1997, Pages 648-652

https://doi.org/10.1006/bbrc.1997.7205 Get rights and content

Abstract

Ob-Ra, b, and e are the major splice forms of the leptin receptor. This study was performed to map the tissue distribution and to quantify the 3 receptor isoforms by heterologous competitive Reverse Transcriptase-Polymerase Chain Reaction (RT-PCR) and RNase Protection Assay (RNase PA). The mRNA of the truncated, membrane bound isoform Ob-Ra was found to be represented ubiquitously. Messenger RNA for the putative functional isoform Ob-Rb could be detected in brain, hypothalamus and in some peripheral tissues (e.g. heart, lung, lymph nodes). The highest ratio between Ob-Rb and Ob-Ra mRNA was found in the hypothalamus, where leptin probably exerts its satiety action. The fact that Ob-Rb mRNA was found in peripheral tissues could indicate possible additional functions of leptin. Transcripts for the shortest splice variant, Ob-Re, which is expected to encode a soluble form of the receptor, were detected in relatively high amounts in many tissues. The levels were comparable to those of leptin mRNA in fat tissue. It is conceivable, therefore, that Ob-Re might be secreted in sufficient amounts to act as a buffering system for freely circulating leptin.

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Citation Excerpt :
These include hypothalamic nuclei, the raphe nucleus, the hippocampus, the amygdala, the ventral tegmental area, the area postrema and the nucleus of the solitary tract [57], and many areas of the brain that contribute to the control of food intake or energy expenditure. Although the level of leptin receptor expression is low in adipocytes, both mRNA [61–63] and protein [64–66] have been detected in various species, demonstrating the presence of long- and short-form receptors [65,66] which were reported to be located on the cell membrane and in small cytoplasmic vesicles in adipocytes [64]. Because adipocytes express leptin receptors [64] and receive sympathetic innervation [67] it is possible for leptin to have direct effects on adipocyte development and function, but also to indirectly modify adipocyte metabolism both through central mechanisms and via modification of release and function of metabolically active hormones, such as insulin.
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Regular ArticleDetection and Quantification of the Leptin Receptor Splice Variants Ob-Ra, b, and, e in Different Mouse Tissues

Abstract

Cell

Anal. Biochem.

Biochem. Biophys. Res. Comm.

Biochem. Biophys. Res. Comm.

Biochem. Biophys. Res. Comm.

FEBS Letters

Blood

J. Biol. Chem.

Biochem. Biophys. Res. Comm.

Neurosc. Lett.

Regular Article
Detection and Quantification of the Leptin Receptor Splice Variants Ob-Ra, b, and, e in Different Mouse Tissues