A Novel Nonenzymatic Pathway for the Generation of Nitric Oxide by the Reaction of Hydrogen Peroxide and D- or L-Arginine

doi:10.1006/bbrc.1997.6428

Biochemical and Biophysical Research Communications

Volume 233, Issue 1, 7 April 1997, Pages 150-153

https://doi.org/10.1006/bbrc.1997.6428 Get rights and content

Abstract

Nitric oxide (NO) is a biologically active molecule known to be enzymatically synthesized from L-arginine in the presence of NO synthetase (NOS). In this study, we demonstrate a novel non-enzymatic pathway for NO synthesis involving hydrogen peroxide and D- or L-arginine. We employed two measures of NO generation. The first consists in the demonstration of the oxidative metabolites of NO (NO₂+ NO₃= NO_X) and the second is the confirmatory finding of chemiluminescence derived from NO. The results show that NO_Xincreases in the incubation mixture containing hydrogen peroxide coupled with D-arginine, L-arginine, L-canavanine, and even the NOS inhibitor N^G-nitro-L-arginine methyl ester (L-NAME). However, chemiluminescence was detected only from the reactions of hydrogen peroxide and D- or L-arginine and was diminished by the addition of carboxy-2-phenyl-4, 4,5,5-tetramethyl-imidazoline-1-oxyl-3-oxide (PTIO), a specific scavenger of NO, confirming NO generation in the reaction.

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¹: To whom correspondence should be addressed at Department of Internal Medicine, Institute of Clinical Medicine, University of Tsukuba 1-1-1 Ten-nodai, Tsukuba, Ibaraki 305, Japan. Fax: 81-298-53-3202. E-mail: [email protected].

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Regular ArticleA Novel Nonenzymatic Pathway for the Generation of Nitric Oxide by the Reaction of Hydrogen Peroxide and D- or L-Arginine

Abstract

Biochem. Biophys. Res. Commun.

Kid. Int.

J. Biol. Chem.

Pharmacol. Rev.

Nature

J. Immunol.

Nature

Nature

Proc. Natl. Acad. Sci. USA

Biochem. J.

Regular Article
A Novel Nonenzymatic Pathway for the Generation of Nitric Oxide by the Reaction of Hydrogen Peroxide and D- or L-Arginine