Amino acid sequence of Phl p 5b 20-mer overlapping peptide sequences used for epitope mapping studies
| Phl p 5b peptide | Sequence |
|---|---|
| 1 | DAGYAPATPAAAGAAAGKAT |
| 2 | PAAAGAAAGKATTEEQKLIE |
| 3 | GKATTEEQKLIEDINVGFKA |
| 4 | KLIEDINVGFKAAVAAAASV |
| 5 | GFKAAVAAAASVPAADKFKT |
| 6 | AASVPAADKFKTFEAAFTSS |
| 7 | KFKTFEAAFTSSSKAAAAKA |
| 8 | FTSSSKAAAAKAPGLVPKLD |
| 9 | AAKAPGLVPKLDAAYSVAYK |
| 10 | PKLDAAYSVAYKAAVGATPE |
| 11 | VAYKAAVGATPEAKFDSFVA |
| 12 | ATPEAKFDSFVASLTEALRV |
| 13 | SFVASLTEALRVIAGALEVH |
| 14 | ALRVIAGALEVHAVKPVTEE |
| 15 | LEVHAVKPVTEEPGMAKIPA |
| 16 | VTEEPGMAKIPAGELQIIDK |
| 17 | KIPAGELQIIDKIDAAFKVA |
| 18 | IIDKIDAAFKVAATAAATAP |
| 19 | FKVAATAAATAPADDKFTVF |
| 20 | ATAPADDKFTVFEAAFNKAI |
| 21 | FTVFEAAFNKAIKESTGGAY |
| 22 | NKAIKESTGGAYDTYKCIPS |
| 23 | GGAYDTYKCIPSLEAAVKQA |
| 24 | CIPSLEAAVKQAYAATVAAA |
| 25 | VKQAYAATVAAAPQVKYAVF |
| 26 | VAAAPQVKYAVFEAALTKAI |
| 27 | YAVFEAALTKAITAMSEVQK |
| 28 | TKAITAMSEVQKVSQPATGA |
| 29 | EVQKVSQPATGAATVAAGAA |
| 30 | ATGAATVAAGAATTAAGAAS |
| 31 | AGAATTAAGAASGAATVAAG |
| 32 | GAASGAATVAAGGYKV |
Each peptide overlaps by 12 amino acids.