Changes in m-RNA levels for metalloproteinases and their inhibitors.
| Metalloproteinase | Healthy Volunteer N | Median (IQR) Relative Quantification (RQ) | Asthma N | Median (IQR) Relative Quantification (RQ) | P value |
|---|---|---|---|---|---|
| MMP 1 | 9 | 0.0015(0.0021) | 16 | 0.0018 (0.0054) | 0.890 |
| MMP 2 | 9 | 0.0065(0.0075) | 17 | 0.0091(0.0122) | 0.367 |
| MMP 7 | 9 | 0.0016(0.0029) | 17 | 0.0040(0.0074) | 0.07* |
| MMP 8 | 9 | 0.00006(0.00007) | 15 | 0.00011(0.00016) | 0.238 |
| MMP 9 | 8 | 0.0163 (0.0157) | 16 | 0.0296(0.0657) | 0.928 |
| MMP 10 | 9 | 0.0002(0.0001) | 16 | 0.0006(0.0050) | 0.461 |
| MMP 11 | 5 | 0.00002(0.00009) | 9 | 0.00006(0.00005) | 0.112 |
| MMP 12 | 9 | 0.0122 (0.0116) | 16 | 0.3348(0.0468) | 0.07* |
| MMP 14 | 9 | 0.1260(0.0348) | 17 | 0.1001(0.1018) | 0.306 |
| MMP 15 | 9 | 0.0312 (0.0012) | 16 | 0.0019(0.0015) | 0.04** |
| MMP 17 | 9 | 0.0002(0.00021) | 13 | 0.0001(0.0002) | 0.40 |
| MMP 19 | 9 | 0.0769 (0.0809) | 17 | 0.1039(0.0662) | 0.491 |
| MMP 25 | 9 | 0.0213(0.0386) | 16 | 0.0734(0.0950) | 0.04** |
| ADAM 8 | 9 | 0.0199(0.0220) | 16 | 0.0530(0.0434) | 0.07* |
| ADAM 9 | 9 | 0.2242(0.0352) | 17 | 0.2381(0.0613) | 0.228 |
| ADAM 10 | 9 | 0.3032(0.0906) | 17 | 0.2415(0.1213) | 0.209 |
| ADAM 12 | 8 | 0.0006(0.0011) | 16 | 0.0009(0.0007) | 1.0 |
| ADAM 17 | 9 | 0.1294(0.0502) | 17 | 0.0712(0.0446) | 0.007** |
| ADAM 19 | 9 | 0.0078(0.0094) | 16 | 0.0165(0.0179) | 0.07* |
| ADAM 28 | 9 | 0.0030(0.0030) | 15 | 0.0106(0.0094) | 0.03** |
| ADAM TS1 | 8 | 0.0026(0.0111) | 14 | 0.0007(0.0006) | 0.764 |
| ADAM TS15 | 9 | 0.0088(0.0077) | 17 | 0.0042(0.0039) | 0.008** |
| TIMP 1 | 9 | 0.3072(0.2163) | 17 | 0.6409(0.5125) | 0.06* |
| TIMP 2 | 9 | 0.7018(0.1670) | 17 | 0.4360(0.2671) | 0.007** |
| TIMP 3 | 9 | 0.009(0.0014) | 17 | 0.0024(0.0011) | 0.164 |
| TIMP 4 | 9 | 0.0012(0.0006) | 16 | 0.0018(0.0047) | 0.357 |
↵p <0.05**, p=0.05–0.07*
Values represent gene of interest expressed as relative quantification (RQ) relative to a housekeeping gene, glyceraldehyde 3-phosphate dehydrogenase (GAPDH) using a 2-ΔCt transformations, ΔCt is the threshold cycle (Ct) of the target gene–Ct of GAPDH.