Sequence alignment of the in silico defined epitope sequence (YAVFEAALT) within Phl p 5b peptide 26 (p26), from Phleum pratense and other commonly occurring grass species allergens in the UK
| Grass allergen | p26 epitope sequence | Percentage sequence alignment |
|---|---|---|
| Phl p 5.0201 | YAVFEAALT | – |
| Dac g 5 isoallergen 2 | YAVFEAALT | 100 |
| Dac g 5 isoallergen 1 | YAVFEAALT | 100 |
| Dac g 5 | YAVFEAALT | 100 |
| Lol p VA | YAVFEAALT | 100 |
| Lol p 5C | YAVFEAALT | 100 |
| Hol l 5.0201 | YAVFEAALT | 100 |
| Hol l 5b | FAVFKAALT | 77 |
| Poa p 5.0201 | FAVFKAALT | 77 |
| Phl p 5.0101 | YTVFETALK | 66 |
| Hol l 5.0101 | YTVFETALK | 66 |
| Lol p 5.0101 | YTVSETALK | 55 |
The TEPITOPE-predicted HLA-DR1-restricted epitope within Phl p 5b-derived p26 (YAVFEAALT) was identified in other grass species. Sequences were aligned using the CLUSTAL 2.0.12 multiple sequence alignment programme available via the European Bioinformatics Institute server: (http://www.ebi.ac.uk/Tools/services/web/toolform.ebi?tool=clustalw2). The Phl p isoallergen investigated in this study is shown in bold. The table highlights the percentage identity of the epitope sequences following calculation of pairwise sequence alignment scores when compared with Phl p 5.0201.
Dac g, Dactylis glomerata; HLA, human leukocyte antigen; Hol l, Holcus lanatus; Lol p, Lolium perenne; Phl p, Phleum pratense; Poa p, Poa pratensis.