Apoptosis induced by recombinant TRAIL and MSC-TRAIL on combination with Vorinostat
| rTRAIL | Vorinostat | rTRAIL and Vorinostat | MSCTRAIL not activated | MSCTRAIL Activated | MSCTRAIL not Activated + Vorinostat | MSCTRAIL Activated + Vorinostat | |
|---|---|---|---|---|---|---|---|
| Ju77 | 7.71% | 51.35% | 80.77% | 10.32% | 48.73% | 47.44%% | 77.7% |
| CRL2081 | 56.75% | 78.95% | 96.6% | 37.3% | 57.63% | 81.45% | 90.93% |
| One58 | 13.41% | 43.97% | 79.27% | 10.88% | 53.8% | 49.25% | 77.8% |
MPM cells are treated with recombinant Trail (100ng/ml) and Vorinostat (2.5µM). MSC are plated in 1:1 ratio with tumour cells. MSC are activated with doxycycline to induce TRAIL expression.