Primary antibodies used for immunohistochemistry
| Antigen | Clone* | Supplier | Antigen retrieval | Dilution† | Against |
|---|---|---|---|---|---|
| CD20 | L26 | Dako, Glostrup, Denmark | Low pH‡ PT | 1:1200 1:600 (IF) | B cells |
| CD4 | 1F6 | Novocastra§, Newcastle Upon Tyne, UK | Low pH‡ PT | 1:50 | T-helper cells |
| CD11c | 5D11 | Novocastra§ | Low pH‡ PT | 1:100 | Macrophages and myeloid dendritic cells |
| CD68 | PG-M1 | Dako | Low pH‡ PT | 1:500 | Monocytes and macrophages |
| CD21 | 1F8 | Dako | Low pH¶ PC | 1:100 | Follicular dendritic cells |
| BDCA-2 | 104C12.08 | Dendritics, Lyon, France | Low pH** PC | 1:50 | Plasmacytoid dendritic cells |
| Multi-Cytokeratin | AE1/AE3 | Novocastra§ | Low pH‡ PT | 1:300 1:150 (IF) | Epithelial cells |
| Ki-67 | Rabbit polyclonal | Biocare Medical, Concord, CA, USA | Low pH** PC | 1:200 | Cell proliferation |
| Langerin (CD207) | 12D6 | Novocastra§ | Low pH‡ PT | 1:100 1:80 (IF) | Langerhans-like dendritic cells |
| Podoplanin | D2–40 | Biocare Medical | Low pH** PC | 1:100 | Lymphatic endothelium |
| α-Smooth muscle actin | 1A4 | Sigma-Aldrich, St Louis, MO, USA | Low pH** PC | 1:1000 | Smooth muscle cells |
*Mouse monoclonal antibodies unless otherwise stated.
†Working dilution for bright field staining, unless otherwise stated.
‡EnVision FLEX Target Retrieval Solution, pH 6.1, K8005, Dako.
§Distributed by Leica Biosystems Newcastle, UK.
¶S1700, pH 6.1, Dako.
**DIVA Decloaker, pH 6, DV2004 MX, Biocare Medical.
IF, immunofluorescence; PC, pressure cook, 2100 Retriever, Prestige Medical Ltd., Blackburn, England; PT, pre-treatment module, Dako, Glostrup, Denmark.