A Media | BL Media | A CSE | BL CSE | A LPS | BL LPS | A CSE & LPS | BL CSE & LPS | |
---|---|---|---|---|---|---|---|---|
NS IL-8 [pg/ml] | 24060±2514 | 34490±3260 | 36193±3755 | 29417±2352 | 34166±3347 | 36969±5019 | 43031±3211* | 43890±5257 |
HS IL-8 [pg/ml] | 28728±2422 | 22903±3500 | 41908±3269 | 27995±4233 | 37298±3052 | 33298±5856 | 39952±3131 | 34095±5634 |
COPD IL-8 [pg/ml] | 32163±2474 | 43768±8730 | 24376±2474* | 21994±4176* | 44092±3247 | 54604±11363 | 20866±1564* | 27180±4680* |
NS IL-6 [pg/ml] | 227±32 | 735±98 | 253±35 | 678±106 | 391±53 | 633±68 | 469±48* | 714±107 |
HS IL-6 [pg/ml] | 289±48 | 518±102 | 285±53 | 418±86 | 447±77 | 487±125 | 536±55* | 884±169* |
COPD IL-6 [pg/ml] | 432±128 | 846±235 | 300±89 | 728±113 | 680±139 | 1012±186 | 324±43 | 578±92 |
IL-8 and IL-6 release from well differentiated bronchial epithelial cell cultures into apical and basolateral compartments after stimulation with PA LPS±pre-treatment with 5% CSE for 24 h. NS: Non-smoker; HS: Healthy Smoker; A: Apical; BL: Basolateral.
Media: No stimulation; CSE: Stimulation with 5% CSE 24 h; LPS: Stimulation with PA LPS 24 h; CSE & LPS: Pre-treatment with 5% CSE 24 h, stimulation with PA LPS 24 h.
↵* Statistically significant difference for soluble mediator release into a given compartment within each study group relative to the equivalent compartment without stimulation; p<0.05.