| Primary antibody | Dilution | Antigen retrieval pretreatment | Clone and manufacturer | Secondary antibody |
| Monoclonal mouse anti-tryptase/AP | 1:3000 | – | MAB1222A (Chemicon; Temecula, California, USA) | – |
| Monoclonal mouse anti-chymase | 1:100 | MW citrate pH6 | CC1 (NovoCastra; Newcastle-upon-Tyne, UK) | HRP-conjugated goat anti-mouse (Vector Laboratories; Burlingame, California, USA) |
| Monoclonal mouse anti-interleukin 9 receptor | 1:30 | MW citrate pH 6 | CD129, AH9R7 310402 (BioLegends; San Diego, California, USA) | Horse anti-mouse IgG*, 1:200 (Vector Laboratories; Burlingame, California, USA) |
| Polyclonal rabbit anti-VEGF | 1:50 | MW citrate pH 6 | A-20 sc-152 (Santa Cruz, California, USA) | Goat anti-rabbit IgG*, 1:200 (Vector Laboratories; Burlingame, California, USA) |
| Polyclonal goat anti-5-LO | 1:50 | MW citrate pH 6 | N-19 sc-8885 (Santa Cruz, California, USA) | Donkey anti-goat IgG*, 1:100, 705-066-147 (Jackson ImmunoResearch; Suffolk, UK) |
| Polyclonal goat anti-LTC4 synthase | 1:200 | MW citrate pH 6 | S-18 sc-22564 (Santa Cruz, California, USA) | Donkey anti-goat IgG*, 1:100, 705-066-147 (Jackson ImmunoResearch; Suffolk, UK) |
| Polyclonal rabbit anti-IgER1A | 1:50 | MW S1700 | 10980-1-AP (Proteintech Group; Chicago, Illinois, USA) | Goat anti-rabbit IgG*, 1:200 (Vector Laboratories; Burlingame, California, USA) |
| Monoclonal mouse anti-renin | 1:100 | MW citrate pH 6 | Swant Scientific (Bellinzona, Switzerland) | Horse anti-mouse IgG*, 1:200 (Vector Laboratories; Burlingame, California, USA) |
| Polyclonal rabbit anti-FGF-2 | 1:50 | MW citrate pH 6 | 147 sc-79 (Santa Cruz, California, USA) | Goat anti-rabbit IgG*, 1:200 (Vector Laboratories; Burlingame, California, USA) |
| Polyclonal rabbit anti-HDC | 1:50 | MW citrate pH 6 | Provided by Professor Lo Persson (Lund University) | Goat anti-rabbit IgG*, 1:200 (Vector Laboratories; Burlingame, California, USA) |
| Polyclonal rabbit anti-c-kit | 1:400 | MW citrate pH 6 | Dako (Glostrup, Denmark) | Goat anti-rabbit IgG*, 1:200 (Vector Laboratories; Burlingame, California, USA) |
For antibody references see online supplement.
High-temperature antigen unmasking techniques (microwave for 2×7 min, 800W) were performed in citrate buffer pH 6 (Merck, Darmstadt, Germany) or S1700 (Dako, Glostrup, Denmark).
*Biotinylated.