Primer | Sequence 5′–3′ | Annealing temp (°C) | Amplicon size (bp) |
GAPDH Fw | GGGCTCTCCAGAACATCATCC | 60 | 130 bp |
GAPDH Rv | GTCCACCACTGACACGTTGG | ||
SCCA Fw | GGCAGCTGCAGCTTCTG | 55 | 80 bp |
SCCA Rv | AGCCGCGGTCTCGTGC | ||
TGFβ Fw | GCCCTGGACACCAACTATTGC | 60 | 161 bp |
TGFβ Rv | AGGCTCCAAATGTAGGGGCAG |
bp, base pair; GAPDH, glyceraldehyde-3-phosphate dehydrogenase; Fw, forward; Rv, reverse; SCCA, squamous cell carcinoma antigen; TGFβ, transforming growth factor β.