Antibodies used for four-colour flow cytometry
| Antigen | Label | Clone | Company | |
|---|---|---|---|---|
| APC, allophycocyanin; BDCA, blood dendritic cell antigen; FITC, fluorescein isothiocyanate; PE, phycoerythrin; PerCP, peridinin chlorophyll protein. | ||||
| Caltag/Invitrogen, Karlsruhe, Germany; Dako, Glastrup, Denmark; Miltenyi Biotec, Bergisch Gladbach, Germany; Immunotods, Friesoythe, Germany. | ||||
| Lineage cocktail | CD3 | FITC | SK7 | BD Biosciences |
| CD14 | FITC | MφP9 | BD Biosciences | |
| CD16 | FITC | 3G8 | BD Biosciences | |
| CD19 | FITC | SJ25C1 | BD Biosciences | |
| CD20 | FITC | L27 | BD Biosciences | |
| CD56 | FITC | NCAM16.2 | BD Biosciences | |
| Other antibodies | CD11c | PE | S-HCL-3 | BD Biosciences |
| CD11c | APC | S-HCL-3 | BD Biosciences | |
| CD123 | PE | 9F5 | BD Biosciences | |
| HLA-DR | PerCP | L243 | BD Biosciences | |
| CD40 | APC | HB14 | Caltag/Invitrogen | |
| CD83 | APC | HB15e | Caltag/Invitrogen | |
| CD86 | APC | BU63 | Caltag/Invitrogen | |
| CD80 | APC | MEM-233 | Immunotools | |
| CD1a | PE | NA1/34 | Dako | |
| BDCA-1 | APC | AD5-8E7 | Miltenyi Biotec | |
| BDCA-2 | APC | AC144 | Miltenyi Biotec | |
| BDCA-3 | APC | AD5-14H12 | Miltenyi Biotec | |
| BDCA-4 | APC | AD5-17F6 | Miltenyi Biotec | |