Primer sequences used in study
| HCM, heavy chain myosin; CPN 1, calponin 1. | |
| αSMA | Forward primer 5′ GACAGCTACGTGGGTGACGAA 3′ |
| Reverse primer 5′ TTTTCCATGTCGTCCCAGTTG 3′ | |
| Probe FAM-5′ TGACCCTGAAGTACCCGATAGAACATGGCT 3′-TAMRA | |
| HCM | Forward primer 5′ GCCTCCGTGCTACACAACCT 3′ |
| Reverse primer 5′ CACGCAGAAGAGGCCAGAG 3′ | |
| Probe FAM-5′ ACGTATATATTAGCCCTGAGAAGTACCGCTCCCTT 3′-TAMRA | |
| CPN 1 | Forward primer 5′ GGTGAAGCCCCACGACATT 3′ |
| Reverse primer 5′ GTTCACCTTGTTTCCTTTCGTCTT 3′ | |
| Probe FAM-5′ TGCAGTCCACCCTCCTGGCTTTGT 3′-TAMRA | |
| Desmin | Forward primer 5′ GGAGAGGAGAGCCGGATCA 3′ |
| Reverse primer 5’GGGCTGGTTTCTCGGAAGTT 3′ | |
| Probe FAM-5′ TCTCCCCATCCAGACCTACTCTGCCCTT 3′-TAMRA | |
| γ-actin | Forward primer 5′ CAGGTTATCACCATTGGCAATG 3′ |
| Reverse primer 5′ ATGAATTCCAGCGGACTCCAT 3′ | |
| Probe FAM-5′ CCCTGAGACCCTCTTCCAGCCTTCCTTT 3′-TAMRA | |