PCR primers and restriction digests
| Polymorphism | Position | Primers (5′–3′) | Annealing temperature(°C) | Detection method | Restriction enzyme | References |
|---|---|---|---|---|---|---|
| N/A, not applicable. | ||||||
| MMP-9 | ||||||
| −1702T>A | Promoter | P13F gcctggcacatagtaggccc | 59 | PCR-SSCP | N/A | Minematsu et al9 |
| P14R cttcctagccagccggcatc | ||||||
| −1562C>T | Promoter | P13F gcctggcacatagtaggccc | 59 | PCR-RFLP | FokI | Minematsu et al9 |
| P14R cttcctagccagccggcatc | ||||||
| −861C>T | Promoter | P9F gtgacataatcatggctcac | 53 | PCR-RFLP | Hsp92II | Minematsu et al9 |
| P10R cttcctctccctgcttcatc | ||||||
| 836G>A (Arg279Gln) | Exon 6 | E6F ctcgccccaggactctacac | 55 | PCR-RFLP | BsoBI | Minematsu et al9 |
| E6R gtggaggtacctcgggtcggg | ||||||
| TIMP-1 | ||||||
| 323C>T (Pro87Pro) | Exon 4 | T1F agacacttcccctcatccatc | 59 | PCR-SSCP | N/A | Genbank D11139 |
| T1R gaccattcccatcagagcctc | ||||||
| 434T>C (Phe124Phe) | Exon 5 | T2F ccaccccaatttcagtctatcg | 64 | PCR-SSCP | N/A | Genbank D11139 |
| T2R gtcagggtccaggcactcact | ||||||
| 536C>T (Ile158Ile) | Exon 6 | T3F ggggctgtcctgggctatgta | 58 | PCR-RFLP | FokI | Genbank D11139 |
| T3R gaggcaggcaggcaaggtgac | ||||||
| 595C>T (Ser178Phe) | Exon 6 | T4F tcattgcttgtggacggac | 54 | PCR-SSCP | N/A | Genbank D11139 |
| T4R gtggacactgtgcaggctt | ||||||