Table 3

Fate of internalised and resecreted 3H-DPPC containing liposomes

20 min internalisation20 min resecretion
NO2 exposure% intracellular label% intracellular label% extracellular label
Values are mean (SE); n=3–4 experiments.
**p<0.01 between all time points of liposome internalisation in the presence of SP-A and the corresponding time point in the absence of SP-A.
††p<0.01 between label in the PC fraction after 20 minutes internalisation and 20 minutes resecretion.
In the absence of SP-A
0 days
    PC80.3 (3.2)††61.1 (2.4)78.6 (3.7)
    Other lipids18.7 (2.9)38.9 (3.1)21.4 (4.2)
3 days
    PC87.0 (3.7)††59.8 (4.1)67.6 (3.2)
    Other lipids12.3 (3.1)40.1 (3.9)32.3 (5.3)
20 days
    PC88.4 (2.5)††56.7 (2.0)66.5 (2.9)
    Other lipids11.5 (0.8)43.3 (1.6)34.4 (4.1)
28 days
    PC90.1 (1.2)††55.4 (2.6)68.1 (5.1)
    Other lipids9.7 (0.8)44.5 (1.8)31.9 (4.0)
In the presence of SP-A
0 days
    PC89.7 (1.4)**87.1 (3.6)92.0 (1.4)
    Other lipids10.1 (1.9)12.0 (0.9)8.1 (2.0)
3 days
    PC94.3 (1.0)**89.7 (4.7)91.8 (1.1)
    Other lipids5.9 (2.2)10.3 (0.9)8.1 (1.2)
20 days
    PC94.9 (2.3)**92.4 (2.5)90.3 (4.1)
    Other lipids4.8 (1.0)7.6 (0.5)9.7 (0.3)
28 days
    PC96.9 (2.1)**88.1 (6.1)93.2 (2.2)
    Other lipids3.0 (0.6)11.9 (5.2)6.8 (0.9)