20 min internalisation | 20 min resecretion | |||
---|---|---|---|---|
NO2 exposure | % intracellular label | % intracellular label | % extracellular label | |
Values are mean (SE); n=3–4 experiments. | ||||
**p<0.01 between all time points of liposome internalisation in the presence of SP-A and the corresponding time point in the absence of SP-A. | ||||
††p<0.01 between label in the PC fraction after 20 minutes internalisation and 20 minutes resecretion. | ||||
In the absence of SP-A | ||||
0 days | ||||
PC | 80.3 (3.2)†† | 61.1 (2.4) | 78.6 (3.7) | |
Other lipids | 18.7 (2.9) | 38.9 (3.1) | 21.4 (4.2) | |
3 days | ||||
PC | 87.0 (3.7)†† | 59.8 (4.1) | 67.6 (3.2) | |
Other lipids | 12.3 (3.1) | 40.1 (3.9) | 32.3 (5.3) | |
20 days | ||||
PC | 88.4 (2.5)†† | 56.7 (2.0) | 66.5 (2.9) | |
Other lipids | 11.5 (0.8) | 43.3 (1.6) | 34.4 (4.1) | |
28 days | ||||
PC | 90.1 (1.2)†† | 55.4 (2.6) | 68.1 (5.1) | |
Other lipids | 9.7 (0.8) | 44.5 (1.8) | 31.9 (4.0) | |
In the presence of SP-A | ||||
0 days | ||||
PC | 89.7 (1.4)** | 87.1 (3.6) | 92.0 (1.4) | |
Other lipids | 10.1 (1.9) | 12.0 (0.9) | 8.1 (2.0) | |
3 days | ||||
PC | 94.3 (1.0)** | 89.7 (4.7) | 91.8 (1.1) | |
Other lipids | 5.9 (2.2) | 10.3 (0.9) | 8.1 (1.2) | |
20 days | ||||
PC | 94.9 (2.3)** | 92.4 (2.5) | 90.3 (4.1) | |
Other lipids | 4.8 (1.0) | 7.6 (0.5) | 9.7 (0.3) | |
28 days | ||||
PC | 96.9 (2.1)** | 88.1 (6.1) | 93.2 (2.2) | |
Other lipids | 3.0 (0.6) | 11.9 (5.2) | 6.8 (0.9) |