|
Transient transfection
|
Permanent transfection
|
Adenoviral transfection
|
Speed | Rapid | Slow | Rapid |
Ease of transfection | Easy for most cells | More difficult | Easy for most cells |
Range of cells | Narrow | Wide | Wide |
Stable lines | No | Yes | No |
Ease of preparing vector | Relatively easy | Relatively easy | Difficult |
% of cells transfected | Small (10–30%) | High (nearly 100% after selection) | High (nearly 100% with large amount of virus) |
Clones | No | Yes | No |
Ability to transfect non-transformed cell lines | Poor | Poor (cells become senescent) | Excellent |
Ability to study protein processing | Poor | Excellent | Good |
Use in dominant negative experiments | Poor | Good | Excellent |
Expression cloning studies | Good | Poor | Poor |
Ability to use transfected cells in animal experiments | Poor | Excellent | Poor |