PCR primers for amplification of the coding region, the intron-exon boundaries, and some 5′ untranscribed region of the ß subunit of the high affinity immunoglobulin E receptor gene
| Primer | Primer sequence | Nucleotide position (5′-3′) | PCR product (bp) |
|---|---|---|---|
| Exons 1, 2 and 3 | |||
| IGER135 | 5′-GGG ACA ATT CCA GAA GAA GGG-3′ | 65–86 | 2303 |
| IGER133 | 5′-GGG CTA AAT GTT TAA CCC ACC G-3′ | 2368–2347 | |
| Exons 4, 5 and 6 | |||
| IGER465 | 5′-CCA CAC CCG CCT TAT TCG TA-3′ | 4270–4289 | 1534 |
| IGER463 | 5′-TTT TAA GGA ATA AAG CTC CAA TTC G-3′ | 5804–5780 | |
| Exon 7 | |||
| IGER75 | 5′-GAG TTT AAT GAC AGA GAG CGT-3′ | 7100–7120 | 406 |
| IGER73 | 5′-AAA TAG AAG GAG CAT ATT AAG GT-3′ | 7506–7484 |