Substance P (SP) immunoreactivity after assay of raw lavage fluid without employing an extraction process
| Non-atopic non-asthma | Atopic non-asthma | Allergen challenged asthma | Unchallenged asthma | ||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| Patient no. | SP-LI pre-extraction (pg/ml) | Patient no. | SP-LI pre-extraction (pg/ml) | Patient no. | SP-LI pre-extraction (pg/ml) | Patient no. | SP-LI pre-extraction (pg/ml) | ||||||
| 1 | 64 | 6 | 70 | 12 | 50 | 24 | 68 | ||||||
| 2 | 332 | 7 | 46 | 13 | 152 | 25 | 72 | ||||||
| 3 | 72 | 8 | 148 | 14 | 192 | 18 | 72 | ||||||
| 4 | 308 | 9 | 48 | 15 | 112 | 19 | 44 | ||||||
| 5 | 60 | 10 | 56 | 16 | 82 | 23 | 112 | ||||||
| 11 | 52 | 17 | 68 | ||||||||||
| 18 | 100 | ||||||||||||
| 19 | 220 | ||||||||||||
| 20 | 82 | ||||||||||||
| 21 | 110 | ||||||||||||
| 22 | 40 | ||||||||||||
| 23 | 100 | ||||||||||||
SP-LI=substance P-like immunoreactivity.
Three asthmatic subjects (nos 18, 19, 23) were studied on two separate occasions (post challenge and unchallenged). No SP immunoreactivity was detected in any samples after extraction and radioimmunoassay or high performance liquid chromatography and radioimmunoassay.