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Human plasma contains a soluble form of CD86 which is present at elevated levels in some leukaemia patients

Leukemia volume 16, pages 865–873 (2002)Cite this article

Abstract

Cell surface expression of CD86 (mCD86) provides an important co-stimulatory signal which profoundly influences immune responses. In this report, we investigated the potential presence of a circulating soluble form of CD86 (sCD86) in normal individuals and patients with acute myeloid leukaemia (AML) or B cell chronic lymphocytic leukaemia (B-CLL). Circulating sCD86 was detected in the plasma of all normal individuals (1.04 ± 0.33 ng/ml, n = 51) and patients analysed. Plasma collected from AML patients in remission (n = 6) contained only low levels of sCD86 but significantly elevated levels (⩾2.65 ng/ml, P < 0.0001) were detected in 10/24 AML patients analysed at the time of presentation or relapse. Significantly elevated levels of sCD86 were also detected in 2/17 B-CLL patients. There was no correlation between sCD86 levels and other clinical parameters. RT-PCR analysis demonstrated that normal monocytes and dendritic cells, as well as isolated AML (n = 2) and B-CLL (n = 4) cells, expressed an alternatively spliced transcript of CD86 which encoded a soluble form absent in normal T, B and NK cells. The finding that a proportion of leukaemia patients contain elevated levels of sCD86 and that at least some leukaemic cells express sCD86 transcript suggests a potential role for sCD86 in modulating mCD86 signalling during the malignant process.

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Acknowledgements

We thank Lisa Haring and James Dekker for their technical assistance and Dr Robert Peach (Bristol-Myers Sqibb) who generously provided the recombinant CD86-Ig for this study. We are grateful to Drs Spearing, Heaton, Gibbons and Ganly for providing patient samples and information. This work was supported by the Canterbury Medical Research Foundation and the New Zealand Lotteries Grant Board.

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Authors and Affiliations

  1. Haematology Research Group, Christchurch Hospital, Christchurch, New Zealand

    BD Hock, S Budhia & JL McKenzie

  2. Haematology Department, Christchurch Hospital, Christchurch, New Zealand

    WN Patton, D Mannari & P Roberts

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Hock, B., Patton, W., Budhia, S. et al. Human plasma contains a soluble form of CD86 which is present at elevated levels in some leukaemia patients. Leukemia 16, 865–873 (2002). https://doi.org/10.1038/sj.leu.2402466

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