Basophils, eosinophils, and mast cells in atopic and nonatopic asthma and in late-phase allergic reactions in the lung and skin☆,☆☆,★
Section snippets
METHODS
Subjects were recruited from the Allergy Clinic, Royal Brompton Hospital, and the London Chest Hospital. The ethics committee at each center approved the study, and all patients gave written consent. All patients were nonsmokers, with no other intercurrent illness. Asthmatic patients were stable at the time of recruitment with an FEV1 greater than 70% of predicted value at each clinic visit. No patient had been treated with inhaled, nasal, or oral corticosteroids for at least 3 months before
Flow cytometry
Analysis of staining on permeabilized peripheral blood leukocytes by flow cytometry confirmed that BB1 was specific for basophils. There was negligible SMFs with eosinophils, neutrophils, lymphocytes, and monocytes (n = 8) stained with BB1 (Table I). In contrast, the total basophil population was BB1+ because there was a significant shift in the fluorescence of the whole population of basophils (Fig 1).
DISCUSSION
The novel findings in this study are (1) the identification of basophils in bronchial biopsy specimens from atopic and nonatopic asthmatic subjects by using a basophil-specific mAb; (2) the observations that there were approximately 10 times fewer basophils in the biopsy specimens from late-phase asthmatic, as compared with late-phase skin, reactions; (3) that the numbers of eosinophils and mast cells were 10 times higher than basophils in asthma biopsy specimens; and (4) the numbers of
Acknowledgements
We thank Dr Philip W. Askenase, Yale University, for his critical review of the manuscript.
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Supported by a grant from the National Asthma Campaign (UK) and funding from Zeneca Pharmaceuticals, UK.
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Reprint requests: A. Barry Kay, MD, Professor and Head, Allergy and Clinical Immunology, Imperial College School of Medicine, National Heart and Lung Institute, Dovehouse St, London SW3 6LY, UK.
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0091-6749/2000 $12.00 + 0 1/1/102686